葛根素预处理与缺血预处理对大鼠心肌缺血再灌注损伤的保护作用

目的: 观察葛根素(PUE)预处理与缺血预处理(IPC)对大鼠心肌缺血再灌注损伤(I/R)的保护作用,以进一步明确PUE药物预处理(PPC)的心肌保护效应.方法: 采用开胸结扎冠状动脉左前降支的方法复制大鼠心肌I/R模型,设立假手术组(sham组)、I/R组、IPC组、PUE组.用红四氮唑染色法测定各组心肌梗死面积,TUNEL法检测心肌细胞凋亡情况,同时测定血清肌酸激酶(CK)活力和血清肌钙蛋白T(TnT)水平.结果: I/R组心肌梗死面积、血清CK活力、TnT浓度以及心肌细胞凋亡指数较sham组显著增高(P＜0.05或P＜0.01),与I/R组比较,PUE组和IPC组心肌梗死面积明显缩小(P＜0.05),血清CK活力、TnT浓度(P＜0.05或P＜0.01)、心肌细胞凋亡指数显著降低(P＜0.01),PUE组与IPC组间各指标差异无明显性(P＞0.05).结论: PUE预处理明显减轻了大鼠心肌I/R,并与IPC的保护效应相似,产生了PPC效应.

The Protective Effect of Puerarin Preconditioning against Myocardial Ischemia-reperfusion Injury in Rats

Objective: To investigate whether puerarin preconditioning has protective effect against myocardial ischemia-reperfusion injury in rats compared with ischemic preconditioning. Methods: Rat heart ischemia-reperfusion injury (I/R) was induced by occluding the left anterior descending coronary artery for 45 minutes and reperfusion for 120 minutes. Fourty-four rats were randomly divided into four groups (11 in each): (1) sham operation (group sham); (2) I/R model only (group I/R) (3) ischemic preconditioning (group IPC, 3 times of ischemia 5 min and reperfusion 5 min prior to I/R), and (4) puerarin (PUE) pretreated group (group PUE, 100mg/kg of PUE were intravenously injected 5 minutes before ischemia) . TTC staining method was used to determine the myocardial infarct size. Cardiac myocytes apoptosis was detected by TUNEL. The activity of creatine kinase (CK) and concentration of troponin T (TnT) in serum were measured simultaneously. Results: Myocardial infarct size, cardiomyocytes apoptosis index, the activity of CK and concentration of TnT in serum of group I/R were obviously higher than those in group sham (P<0.05 or P<0.01). Compared with group I/R, all these parameters were significantly reduced in IPC and PUE pretreated groups (P<0.05 or P<0.01). Furthermore, there was no significant difference between IPC and puerarin pretreated groups in all these parameters (P>0.05). Conclusions: Puerarin preconditioning (administrating puerarin before ischemia) can significantly reduce myocardial ischemia-reperfusion injury in rats, and its proctective effect is similar to that of IPC.