通心络对糖尿病动脉粥样硬化大鼠Rho/Rho激酶底物影响的研究

目的：观察糖尿病合并动脉粥样硬化大鼠Rho/Rho激酶和血管内皮功能的变化及两者之间的关系,探讨通心络对此变化的影响及可能机制。方法：对Wistar大鼠采用链脲佐菌素诱导制成糖尿病模型,再予以高脂饮食制备糖尿病合并动脉粥样硬化模型,并随机分为模型组和通心络治疗组,普通饮食喂养大鼠作为对照组。药物干预6周后,行血脂、一氧化氮合酶（eNOS）、内皮素（ET-1）及肌球蛋白亚基（MYPT）的检测。结果：模型组大鼠与对照组相比,eNOS降低,ET-1升高,MYPT的表达增加（均P〈0.05）;血脂各项指标除HDL-C明显降低外均增高（均P〈0.05）。通心络治疗组与模型组相比,eNOS升高,ET-1降低,MYPT表达降低（均P〈0.05）;TG降低（均P〈0.05）,TC、LDL-C降低及HDL-C升高（P〉0.05）。结论：糖尿病合并动脉粥样硬化大鼠出现内皮功能异常,通心络可以改善血管内皮功能,其机制可能是通过Rho/Rho激酶信号通路抑制血管内皮损伤。

Effects of Tongxinluo on substrate of Rho/Rho kinase in diabetic rats with atherosclerosis

Objective： To observe the expression of Rho kinase and the change of endothelial function in atherosclerotic rat with diabetes mellitus（DM）,as well as their relationship,and to explore the effect of Tongxinluo on it and its possible mechanism. Methods： Wistar rats were injected STZ to build diabetes mellitus models,and then given hyperlipid diet to build atherosclerotic models. Successful model rats were randomly divided into model group and Tongxinluo treatment group. The control group was given common diet. 6 weeks later,serum lipid,eNOS（endothelial nitric oxide synthase）,ET-1（endothelin）,MYPT（myosine phosphatae targeting subunit） were detected. Results： Compared with control group,serum lipid except for HDL-C,ET-1,and the expression of MYPT increased in model group（all P 〈 0. 05）,eNOS decreased in model group（P 〈 0. 05）. Compared with model group,ET-1,TG and the expression of MYPT decreased significantly in Tongxinluo treatment group（ all P 〈0. 05）,eNOS were increased（P 〈 0. 05）,TC and LDL-C only had a decreasing tendency（all P 〉 0. 05）,HDL-C only had a increasing tendency（P 〉 0. 05）. Conclusion： DM rats with atherosclerosis occur disorders of endothelial function. Tongxinluo has influence on the function of endothelium,and the mechanism may be achieved by intervention of Rho /Rho Kinase’s expression to inhibit the vascular endothelial injury.