脑心通对局灶性脑缺血再灌注损伤大鼠的保护作用

目的 研究脑心通对局灶性脑缺血再灌注损伤后大鼠的保护作用。方法 随机将健康雄性SD大鼠30只分为假手术组（A组）、缺血组（B组）、脑心通治疗组（C组），每组10只,B组和C组采用大脑中动脉线栓法制备大鼠局灶性脑缺血再灌注模型,采用免疫组织化学法和Western blot法检测脑组织中小胶质细胞的激活及人白细胞分化抗原68（CD68）、白细胞介素-1β（IL-1β）、肿瘤坏死因子-α（TNF-α）的表达。结果 脑缺血灶范围内CD68、TNF-α、IL-1β的表达，B组明显高于A组,C组明显低于B组。结论 脑心通通过抑制脑缺血再灌注损伤后炎症反应，对脑缺血大鼠有一定程度的神经保护作用。

Protective effect of Naoxintong on reperfusion after focal cerebral ischemia in rats

Objective     To investigate the protective effect of Naoxintong on reperfusion after focal cerebral ischemia in rats and explore effective methods to interfere with inflammation following acute cerebral ischemia-reperfusion injury. Methods     Thirty male SD rats were randomly divided into three groups: the sham operation group(group A, n=10), the ischemic group (group B, n=10), and the Naoxintong group(group C, n=10). Group B and C had focal cerebral ischemia-reperfusion produced by right middle cerebral artery occlusion. Activation of microglia， and expressions of CD68，IL-1β and TNF-α were detected by immunohistochemistry and Westernblot． Results    Expressions of CD68, IL-1β and TNF-α were more markedly increased in group B than those in group A, and significantly lower in group C than those in group B． Conclusion    Naoxintong can inhibit inflammation reaction after cerebral ischemia, which can provide an inhibitory effect on cerebral ischemia reperfusion injury in rats.