黄芪在同种移植排斥中对CD4+ CD25+ T细胞及Foxp3的影响

探讨黄芪(AST)在同种移植排斥中对CD4+CD25+T细胞动态变化及Foxp3表达的影响。方法：C57BL/6和BALB/c小鼠分别为供体和受体，建立皮肤移植模型。对照组：术后注射生理盐水；AST组：术后给予黄芪注射液；AST+脾细胞(SP)组：术前输注供体鼠脾细胞，术后给予黄芪注射液，CsA+SP组：术前输注供体鼠脾细胞，术后给予环孢素A（CsA）。术后逐日观察移植皮肤存活情况及小鼠一般状况；免疫荧光染色流式细胞术检测脾组织中CD4+CD25+T细胞动态变化；RT PCR检测Foxp3mRNA表达情况。结果：与对照组相比，AST组、AST+SP组、CsA+SP组移植皮片存活时间明显延长（P＜0.05），移植后14?d，CD4+CD25+T细胞数量及Foxp3的相对表达量显著增多（P＜0.05）。结论：黄芪体内用药可延长同种移植物的存活时间，上调CD4+CD25+T细胞及Foxp3的活性。

Effect of Astragalus on CD4+ CD25+ T cells and Foxp3 in allografted mice

［ABSTRACT］Objective: To study the effect of Astragalus (AST) on CD4+CD25+ T cells and Foxp3 after allo transplantation. Methods: Skin of C57BL／6 mice were transplanted to BALB/c mice. Control group: Sodium Chloride were given, group AST: Astragalus injection were given, group AST+spleen cells (SP): spleen cells of donor mice were given before transplantation and Astragalus injection were given after transplantation, group CsA+SP: spleen cells of donor mice were given before transplantation and Ciclosporin（CsA） were given after transplantation. Survival condition of grafted skin and general condition of mice were observed daily; Numbers of CD4+CD25+ T cells were examined by immunofluorescence staining and flow cytometry; The ｍRNA expression of Foxp3 was tested by RT PCR. Results: Compared with group Control, allograft survival time of group AST、 AST+SP、 CsA+SP prolonged significantly（P＜0.05）, The level of CD4+CD25+ T cells and the expression of Foxp3 ｍRNA increased obviously on 14th day after transplantation（P＜0.05）. Conclusion: Using Astragalus in vivo can prolong graft survival time obviously and up regulate the level of CD4+CD25+ T cells and the expression of Foxp3.