两个基因多态性并存与2型糖尿病大血管病变的关系

目的探讨抵抗素+299G/A，PPARγ2 Pro12Ala基因多态性并存与糖尿病大血管病变的关系。方法应用聚合酶链反应 限制性片段长度多态性技术检测296例两个基因多态位点的等位基因、基因型。结果①A组抵抗素基因+299G/A等位基因频率和B组等位基因频率及基因型分布与对照组比较差异均有统计学意义(P＜0.05)。PPARγ2基因 Pro/Ala多态位点的等位基因频率与基因型在2型糖尿病组与对照组间比较差异无统计学意义(P＞0.05)，B组与A组及对照组比较差异均有统计学意义(P＜0.05)； ②多态位点联合分析表明，同时携带GA/AAPA基因型的T2DM患者患大血管病变风险明显低于仅携带一种或者未携带基因型者(P＜0.05)。结论①抵抗素基因+299G/A多态位点的A等位基因及AA基因型，PPARγ2基因 Pro/Ala多态位点的A等位基因可能是大血管病变的保护因素；②同时携带GA/AAPA基因型的T2DM患者大血管病变患病风险更低。

Association between two concurrent gene polymorphisms and type 2 diabetes with macro-angiopathy

To investigate the correlation between polymorphisms of both +299G/A at resistin gene and Pro12Ala at PPARγ2 gene and diabetic macro-angiopathy. MethodGenotypes and alleles of polymorphisms of both genes were determined with polymerase chain reaction restriction fragment length polymorphism assay (PCR RFLP) of 296 subjects in Han Chinese. Results Both resistin gene +299G/A alleles frequencies in group A (the type 2 diabetes group) and alleles frequencies and genotypes in group B(the type 2 diabetes combined with the macro angiopathy group) were statistically significant from those in the control group(P＜0.05), but P/A alleles frequencies and genotypes to of the PPARγ2 gene were statistically different in groups A (P＞0.05 vs the control group) and were statistically different in group B (P＜0.05 vs the control and the A group). Type 2 diabetes with the coexistence of the A allele/AA genotype of resistin gene and the A allele/PA genotype of the PPARγ2 gene had a lower incidence of macro-angiopathy than those with one gene alone or without the gene(P＜0.05). ConclusionThe A allele/AA genotype at resistin gene of +299 and the Ala allele at the PPARγ2 gene of Pro12Ala are related to diabetic macro angiopathy.