| 宫外孕组织沙眼衣原体感染的研究 |
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| 引用本文： | 周宁,于萍,徐增祥,李福民,克丙申,杨道理,谢智农,陈诵芬.宫外孕组织沙眼衣原体感染的研究[J].山东大学学报(医学版),1997(1). |
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| 作者姓名： | 周宁 于萍 徐增祥 李福民 克丙申 杨道理 谢智农 陈诵芬 |
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| 作者单位： | 济南军区总医院，山东医科大学生理学教研室 |
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| 摘 要： | 建立检测沙眼衣原体的ＰＣＲ方法，计算机辅助设计的引物位于编码沙眼衣原体主要外膜蛋白（ＭＯＭＰ）的基因区内，扩增产物为１４４ｂｐ。该引物具有高度的特异性和敏感性，其最小检出量为５ｆｇ。应用该引物检测了５２份宫外孕组织中沙眼衣原体ＤＮＡ，测得宫外孕患者沙眼衣原体的阳性率为４０．４％，明显高于对照组的１２．５％（Ｐ＜０．０１），从ＤＮＡ水平证实在宫外孕组织内存在沙眼衣原体
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| 关 键 词： | 沙眼衣原体 妊娠，异位 聚合酶链反应 脱氧核糖核酸 |
DETECTION OF CHLAMYDIA TRACHOMATIS DNA FROM SALPINGIAN TISSUE OF ECTOPIC PREGNANCY |
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| Zhou Ning Yu Ping Xu Zengxiang,et al.DETECTION OF CHLAMYDIA TRACHOMATIS DNA FROM SALPINGIAN TISSUE OF ECTOPIC PREGNANCY[J].Journal of Shandong University:Health Sciences,1997(1). |
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| Authors: | Zhou Ning Yu Ping Xu Zengxiang |
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| Abstract: | The PCR assay for detection of chlamydia trachomatis (CT) DNA was developed.The oligonucleotied primer designed with computer was located at major outer membrane protein gene sequence of chlamydia trachomatis,it's amplified products was 144bp.The CT PCR assay had high specificity and sensitivity,the 5fg CT DNA could be detected.Using this primer,CT DNA in 52 salpingian tissues of ectopic pregnancy was detected to study the relationship between ectopic pregnancy and chlamydia trachomatis,and to compare with traditional cell culture.The positive percentage of ectopic pregnancy patients was 40.4%(21/52) which was significantly higher than that of control group(12.5%).It is first proved that chlamydia trachomatis is present in ectopic pregnancy tissues by DNA detection. |
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| Keywords: | Chlamydia trachomatis Pregnancy ectopic Polymerase chain reaction DNA |
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