| 化合物N-2035对大鼠局灶性脑缺血再灌注损伤的保护作用 |
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| 引用本文： | 孙茹,张岫美,魏欣冰,王姿颖,孙霞,光红梅.化合物N-2035对大鼠局灶性脑缺血再灌注损伤的保护作用[J].山东大学学报(医学版),2003,41(3):249-252. |
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| 作者姓名： | 孙茹 张岫美 魏欣冰 王姿颖 孙霞 光红梅 |
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| 作者单位： | 山东大学医学院药理学研究所 |
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| 摘 要： | 目的:观察异黄酮类化合物N-2035对大鼠局灶性脑缺血再灌注损伤的保护作用。方法:利用大脑中动脉栓线阻断法(MCAO)制备大鼠局灶性脑缺血再灌注损伤模型,观察化合物N-2035对缺血再灌注大鼠的脑水肿、细胞内钙及凋亡抑制基因bcl-2表达的影响。结果:化合物N-2035能改善局灶性脑缺血再灌注损伤脑水肿,降低细胞内钙,增加bcl-2的表达。结论:化合物N-2035对大鼠局灶性脑缺血再灌注损伤具有保护作用,其作用机制可能与化合物N-2035抗细胞凋亡有关。
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| 关 键 词： | 化合物N-2035 脑缺血 再灌注损伤 细胞凋亡 细胞内钙 基因 bcl-2 大鼠 近交系 |
| 文章编号： | 1671-7554(2003)03-0249-04 |
| 修稿时间： | 2002年11月4日 |
The protective effect of compound N-2035 on focal brain ischemia-reperfusion injury in rats |
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| SUN Ru,ZHANG Xiu-mei,WEI Xin-bing,et al.The protective effect of compound N-2035 on focal brain ischemia-reperfusion injury in rats[J].Journal of Shandong University:Health Sciences,2003,41(3):249-252. |
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| Authors: | SUN Ru ZHANG Xiu-mei WEI Xin-bing |
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| Abstract: | Objective:To investigate the protective effect of compound N-2035obtained from puerarin,a kind of isoflavone that was extracted from Chinese traditional medicine on focal brain is-chemia-reperfusion injury in rats.Methods:The models of focal brain ischemia reperfusion injury by middle cerebral artery occlusion(MCAO)were established in Wister rats.Cerebral edema was measured by dry /wet mass method and intracellular calcium and the expression of bcl-2was examined by flow cytometer.Re sults:N-2035in a dose of40mg /kg positively relieved cerebral edema;each dose de-creased intracellular calcium and increased the expression of bcl-2.Con clusion:Compound N-2035can relieve brain damage induced by focal ischemia /reperfusion in rats.The mechanism may be related with the decrease of intracellular calcium and the increase of the expression of bcl-2. |
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| Keywords: | Compound N-2035 Cerebral ischemia Reperfusion damage Apoptosis Intracellu-lar calcium Gene bcl-2 Rats inbred strains |
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