| 重组SOD的摇瓶发酵条件研究 |
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| 引用本文： | 陈航,黄晓南,陈春,刘树滔,饶平凡.重组SOD的摇瓶发酵条件研究[J].医学理论与实践,2006,19(11):1255-1257. |
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| 作者姓名： | 陈航 黄晓南 陈春 刘树滔 饶平凡 |
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| 作者单位： | 福州大学生物工程研究所,福建省福州市,350002 |
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| 摘 要： | 目的:对含PTD-SOD重组质粒的工程菌进行诱导表达。方法:通过SOD活性和SDS-PAGE电泳分析,考察了诱导时间和诱导剂浓度对PTD-SOD表达量的影响。同时考察了诱导温度对产物表达形式的影响。结果:表达条件为IPTG浓度0.5mM,诱导时间4h,诱导温度20℃。结论:在上述条件下重组SOD在摇瓶中的表达效果最好。
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| 关 键 词： | 超氧化物歧化酶 诱导剂 基因表达 发酵 大肠杆菌 |
| 文章编号： | 1001-7585（2006）11-1255-03 |
| 收稿时间： | 2006-04-25 |
| 修稿时间： | 2006年4月25日 |
Effect of Shaking-flask Conditions on Expression of PTD-SOD by Escherichia Coli |
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| CHEN Hang, HUANG Xiaonan, CHEN Chun.Effect of Shaking-flask Conditions on Expression of PTD-SOD by Escherichia Coli[J].The Journal of Medical Theory and Practice,2006,19(11):1255-1257. |
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| Authors: | CHEN Hang HUANG Xiaonan CHEN Chun |
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| Institution: | Institute of Biotechnology, Fuzhou University, Fujian Province 350002 |
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| Abstract: | Objective:To study the induction and expression of strains with recombinant plasmid of PTD-SOD.Methods: The effects of the concentration of inducer as well as the inducing time on the expression amount of PTD-SOD were investigated by SDS-PAGE analysis.The influence of induction temperature on the style of expressed PTD-SOD was also analyzed.Results:The expression conditions were as follows: IPTG concentration 0.5mM,induction temperature 20℃ and induction time 4 hours.Conclusion: The above conditions are the optimum expression conditions for recombinant strain flask cultivation. |
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| Keywords: | PTD-SOD Inducer Gene expression Fermentation Escherichia coli |
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