人巨细胞病毒UL141片段在临床低传代分离株中的多态性研究

目的:研究人巨细胞病毒不同临床分离株UL141基因多态性,并分析这种多态性与人巨细胞病毒先天感染疾病之间的关系.方法:对荧光定量PCR方法检测HCMV-DNA阳性的临床低传代分离株进行UL141基因全序列PCR扩增,对扩增阳性的标本进行测序及分析.结果:19株临床低传代分离株在Toledo株UL141基因227位均缺失一个碱基T,因此产生两个新的ORF(UL141A和UL141B).与Toledo株相应片段比较,UL141A预测蛋白质第75位氨基酸后序列和翻译后修饰位点产生大量变异.UL141B高度保守.结论:临床分离株的UL141片段产生两个新的ORF.

Polymorphism of human cytomegalovirus UL141 gene in clinical isolates of low passage

Objective: To study the polymorphism of human cytomegalovirus(HCMV) UL141 in clinical isolates and find the relationship between the polymorphism and the outcome of congenital HCMV infection.Methods: PCR was performed to amplify the entire UL141 region of clinical isolates,which had been proven HCMV-DNA positive by real time PCR.The PCR products were sequenced and the data were analyzed.Results:There was a nucleotide deletion at the position 227 in clinical isoltes,and 2 new UL141 open reading frames named UL141A and UL141B were found.In clinical isolates the predicted proteins of UL141A were hypervariable post the position 75 of amino acid sequence.The predictied protein of UL141B was highly conserved.Conclusion:There are two UL141 open reading frames in clinical isolates.