脊髓型减压病大鼠血清血管内皮生长因子含量的变化

目的]通过制作脊髓型减压病大鼠模型，观察成年大鼠脊髓受损后不同时期血管内皮生长因子（VEGF）含量的变化。方法]健康雄性SD大鼠24只，鼠龄120～250 d ，体质量200～250 g ，24 h昼夜循环光照，自由饮水、取食，室温（24±2）℃。所有动物术前均经 Y 型电迷宫测试，然后随机分为三组，每组各8只。第一组：正常对照组；第二组：安全减压组；第三组：减压病组。各组分别于出舱后1d、1周、2周、4周和8周进行血清VEGF含量检测。结果]正常对照组，安全减压组显示出舱后1 d血清VEGF含量较少，减压病组血清VEGF含量较多，与正常对照组，安全减压组相比差异有显著性（ P ＜0．05），减压病组血清V EG F含量1周后下调，1周、2周减压病组与正常对照组，安全减压组相比差异亦有显著性（ P ＜0．05），4周、8周时各组比较差异均无显著性（ P ＞0．05）。结论]脊髓型减压病脊髓损伤可上调血清VEGF含量，从而对脊髓损伤起到指导治疗作用。

Changes of Serum Vascular Endothelial Growth Factor in Spinal Cord Decompression Sicknessin Rats

Objective]To observe the change of vascular endothelial growth factor（VEGF） in adult rats after spinal cord injury at different period through establishing a rat model of spinal cord decompression sickness. Meth- ods]Twenty four healthy male SD rats aged 12-250d and weighed 200-250g received 24-hour circadian light, free drinking and food under room temperature 24：52 2℃. All animals underwent Y-type maze test before operation, and then were randomly divided into normal control group（ n =8）, safe decompression group（ n =8） and decompression sickness group（ n =8）. Serum VEGF of rats in each group 1d, 1w, 2w, 4w and 8w after out of cabin was detected. Results] Serum level of VEGF in normal control group and safe decompression group ld after out of cabin was low- er, while that in decompression sickness group was higher, and there was significant difference between normal con- trol group and safe decompression group（ P 〈0.05）. Serum level of VEGF in decompression sickness group lw after out of cabin decreased, and there was significant difference between decompression sickness group lw and 2w after out of cabin and safe decompression group（ P 〈0. 05）, but there was no significant difference among 3 groups at 4w and 8w（ P 〉0.05）. Conclusion]Spinal cord injury in decompression sickness can increase serum VEGF, which may guide the treatment of spinal cord injury.