顺铂联合吡柔比星对脑胶质瘤U87细胞株增殖的抑制作用

目的 观察顺铂（CDDP）联合吡柔比星（THP）对脑胶质瘤U87细胞株增殖的抑制作用.方法 采用MTT法检测不同浓度CDDP、THP以及两者联合应用时对U87细胞增殖作用的影响.结果 随药物浓度增加,CDDP组、THP组和联合组对U87细胞增殖抑制作用逐渐增强（均P＜0.01）.联合组对U87细胞增殖抑制率较同浓度CDDP组、THP组明显增加（P＜0.05或P＜0.01）.当CDDP和THP浓度为≤0.625μg/mL时,联合组Q值分别为1.72、1.31,两药联合应用具有协同作用,当CDDP和THP浓度≥1.25 μg/mL时,联合组Q值分别为0.94、0.88、0.86、0.89,两药联合应用具有相加作用.结论 CDDP联合THP较单纯的CDDP或THP抑制U87细胞增殖作用更强,具有浓度依赖性.在较低剂量下就能达到明显肿瘤抑制效应,对杀伤U87细胞具有确切协同效应.

Inhibition effect of cis-diaminedichloroplatinum （CDDP） combined with pirarubicin （THP） on cell proliferation of brain glioma U87

Objective To discuss inhibition effect of cis-Diaminedichloroplatinum （CDDP） combined with Pirarubicin （THP） on cell proliferation of brain glioma U87. Methods The MTT method was applied to detect the cell proliferation of brain glioma U87 in the influence by CDDP, THP and the combination of both under different concentration. Results As the concentration of drugs rose, the influences on inhibition of cell proliferation of brain glioma U87 in CDDP group, THP group and combination group were enhanced gradually （P〈0.01 on average）. Compared with CDDP group and THP group under the same concentration, the inhibitory rate of cell proliferation obviously rose （P〈0.05 or P〈0.01）. When the concentration in CDDP group and THP group was no more than 0.625μg/mL, the Q value in combination group were 1.72 and 1.31 respectively, which showed the coordination effect of combination of these two drugs. When the concentration in CDDP group and THP group was no less than 1.25txg/mL, the Q value in combination group were 0.94, 0.88, 0.86 and 0.89 respectively, which showed the additive effect of combination of these two drugs. Conclusion Compared with separate CDDP or THP, the combination of both CDDP and THP has more favorable inhibition of cell proliferation of brain glioma U87, with concentration dependent, which can achieve obviously tumor suppression effect under low dose, and has definitive coordination effect to kill and wound U87 cells.