| MP-DNA荧光定量聚合酶链反应检测在儿童肺炎支原体肺炎中的诊断价值 |
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| 引用本文： | 吴宏图,单春明,周杨杨.MP-DNA荧光定量聚合酶链反应检测在儿童肺炎支原体肺炎中的诊断价值[J].陕西医学杂志,2011,40(1):50-52. |
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| 作者姓名： | 吴宏图 单春明 周杨杨 |
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| 作者单位： | 江苏省淮安市第二人民医院儿科,淮安,223002 |
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| 摘 要： | 目的:探讨肺炎支原体DNA(MP-DNA),支原体抗体(MP-Ig),C反应蛋白(CRP)对诊断支原体肺炎的敏感性和特异性,为临床提供准确快速的诊断依据。方法:采用MP荧光定量PCR法检测患儿呼吸道分泌物标本中的MP-DNA水平,同时检测血清CRP和MP-IgM的浓度,同时相互进行比较。结果:MP-DNA和MP-Ig对诊断支原体肺炎的敏感性均高于CRP,三个指标中敏感性以MP-Ig最高;特异性和阳性预测值以MP-DNA最高;阴性预测值和约登指数,MP-DNA和MP-Ig两者相差无几。结论:MP-DNA检测对肺炎支原体的早期诊断有一定价值。
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| 关 键 词： | 肺炎支原体/诊断 脱氧核糖核酸/代谢 C反应蛋白/代谢 聚合酶链反应 儿童 |
Diagnostic value of MP-DNA fluorescent quantitative polymerase chain reactiondetection in children with mycoplasma pneumoniae pneumonia |
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| Wu Hongtu,Shan Chunming,Zhou Yangyang.Diagnostic value of MP-DNA fluorescent quantitative polymerase chain reactiondetection in children with mycoplasma pneumoniae pneumonia[J].Shaanxi Medical Journal,2011,40(1):50-52. |
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| Authors: | Wu Hongtu Shan Chunming Zhou Yangyang |
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| Institution: | Wu Hongtu Shan Chunming Zhou Yangyang Department of Pediatrics,the Second People's Hospital of Huaian City,Jiangsu Province(Huaian 223002) |
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| Abstract: | Objective: To explore diagnostic evaluation of sensitivity and specificity of MP-DNA,MP-Ig are CRP in children with MP pneumonia, and provide accurate and quick diagnostic proof to clinic medicals.Methods: children's respiratory secretion samples were collected for fluorescent quantitative PCR for MP-DNA,and the serum's concentrations of CRP and MP-IgM were detected by immunobiochemistry methods,and the three indexes were compared with each other.Results: MP-DNA and MP-Ig were superior for sensitivity in di... |
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| Keywords: | Mycoplasma Pneumoniae/diagnosis DNA/metabolism Creactive protein/metabolism Polymerase chain reaction Child |
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