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| pcDNA3.1/ASIC1a真核表达载体的构建及其在佐剂性关节炎大鼠关节软骨细胞中的表达 | |
| 引用本文： | 王念,陈飞虎,江晟,荣超,胡伟,吴繁荣,沈际佳.pcDNA3.1/ASIC1a真核表达载体的构建及其在佐剂性关节炎大鼠关节软骨细胞中的表达[J].安徽医科大学学报,2011,46(6):514-518. |
| 作者姓名： | 王念 陈飞虎 江晟 荣超 胡伟 吴繁荣 沈际佳 |
| 作者单位： | 1. 安徽医科大学药学院,合肥,230032 2. 安徽医科大学第二附属医院药剂科,合肥,230601 3. 安徽医科大学基础医学院,合肥,230032 |
| 摘 要： | 目的 通过构建真核表达pcDNA3.1/ASIC1a质粒,转染佐剂性关节炎(AA)大鼠关节软骨细胞,建立酸敏感通道在关节软骨细胞中过表达的模型,观察ASIC1a mRNA及其蛋白的相对表达.方法 通过PCR扩增目的 基因ASIC1a,并用XbaⅠ和BamHⅠ进行双酶切,同时用这两种酶双酶切质粒pcDNA3.1,将其酶切... |
| 关 键 词： | 关节炎 实验性 软骨细胞 脂质体 转染 |
Construction of pcDNA3.1/ASIC1a eukaryotic expression vector and its expression in articular cartilage cells of adjuvant arthritis rats |
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| Wang Nian,Chen Feihu,Jiang Shen,et al.Construction of pcDNA3.1/ASIC1a eukaryotic expression vector and its expression in articular cartilage cells of adjuvant arthritis rats[J].Acta Universitis Medicinalis Anhui,2011,46(6):514-518. | |
| Authors: | Wang Nian Chen Feihu Jiang Shen |
| Institution: | Wang Nian,Chen Feihu,Jiang Shen,et al(School of Pharmacology,Anhui Medical University,Hefei 230032) |
| Abstract: | Objective To construct eukaryotic expression vector of acid sensing ion channel-1a(ASIC1a),and transfect it into the articular cartilage cells of adjuvant arthritis(AA) rats to make the model of overexpression of ASIC1a.Methods Through PCR,recyling and purification of gelatin,connecting with vectors,translation into agarose electrophoresis,competent bacteria,the collection of plasmid and the identification of double enzyme cutting to contrast the plasmid to be used for transfection.We used lipofectamine 2 0... |
| Keywords: | arthritis experimental cartilage cells liposomes transfection |
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