| 人胚神经干细胞的分离培养和鉴定 |
| |
| 引用本文： | 罗树伟,谢常青,卢光琇.人胚神经干细胞的分离培养和鉴定[J].中南大学学报(医学版),2004,29(2):129-131. |
| |
| 作者姓名： | 罗树伟 谢常青 卢光琇 |
| |
| 作者单位： | 中南大学湘雅医学院人类生殖工程研究室，湖南省干细胞工程技术研究中心，长沙 410078 |
| |
| 基金项目： | 国家自然科学基金重点项目 (30 0 30 0 70 )，国家自然科学基金 (30 1 0 4 0 30 4 8)，湖南省自然科学基金(OOJJY2 0 36)，湖南省科技攻关项目 (0 1 99Y1 0 0 1 ) |
| |
| 摘 要： | 目的：从流产人胚前脑中分离培养人胚神经干细胞。方法：神经干细胞具有自我复制和多向分化潜能。本实验利用神经干细胞特定的培养增殖培养体系分离纯化人胚神经干细胞，并对分离得到的神经干细胞的分子标记及分化潜能进行鉴定。结果：从8~10周龄流产人胚胎分离得到了神经干细胞，它能表达神经干细胞特异性抗原nestin，并能在体外诱导分化为神经元细胞和神经胶质细胞。结论：在人胚中枢神经系统中存在神经干细胞，并能够在体外扩增，为神经干细胞的基础研究和临床应用奠定了基础。
|
| 关 键 词： | 神经干细胞 分离 鉴定 人胚 |
| 文章编号： | 1672-7347(2004)02-0129-03 |
| 收稿时间： | 2003-04-01 |
| 修稿时间： | 2003年4月1日 |
Isolation culture and identification of human neural stem
cells from human embryos |
| |
| LUO Shu wei,XIE Chang qing,LU Guang xiu.Isolation culture and identification of human neural stem
cells from human embryos[J].Journal of Central South University (Medical Sciences)Journal of Central South University (Medical Sciences),2004,29(2):129-131. |
| |
| Authors: | LUO Shu wei XIE Chang qing LU Guang xiu |
| |
| Institution: | Human Reproductive Engineering Research Center, Xiangya School of Medicine , Central South University,
Hunan Stem Cells Research Center, Changsha 410078, China |
| |
| Abstract: | OBJECTIVE: To obtain and culture the human neural stem cells from the aborted human embryos. METHODS: The neural stem cells were isolated and purified by the specific proliferous culture system of neural stem cells, and the molecular maker and multi-potency of the obtained neural stem cells were identified. RESULTS: Human neural stem cells, which were isolated from 8 - 10 month old aborted human embryos, could express nestin ( a kind of specified antigen of the neural stem cell), and it could be differentiate into neurons and glials. CONCLUSION: Neural stem cells can exist in human embryos, and it can be expanded in vitro. |
| |
| Keywords: | neural stem cells isolation identification human embryo |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《中南大学学报(医学版)》浏览原始摘要信息 |
| 点击此处可从《中南大学学报(医学版)》下载免费的PDF全文 |
