bFGF与VEGF复合磷酸钙骨水泥修复 兔桡骨缺损的实验研究

目的：探讨碱性成纤维细胞生长因子(bFGF)与血管内皮生长因子(VEGF)促进骨生长是否具有协同作用。方法：36只家兔的双侧桡骨均建立1.0 cm的骨缺损实验模型后随机分为A，B，C，D组。其中A组为对照组，于骨缺损部位植入单纯磷酸钙骨水泥(CPC)；B组植入复合bFGF的CPC，C组植入复合VEGF的CPC，D组植入联合应用bFGF和VEGF的CPC。分别于术后3，6，12周切取标本，通过X线摄片、骨密度测量、生物力学测试、组织形态学观察等手段，观察各个时期新骨形成的情况。结果：X线摄片显示12周时D组骨缺损完全修复，材料基本降解；B，C组骨缺损基本修复。骨密度测量显示术后12周时D组骨密度明显高于B，C两组(P＜0.05)。生物力学测试(12周时)显示D组最大抗折载荷明显高于B，C两组(P＜0.05)。组织形态学观察显示12周时，编织骨改建为成熟的板层骨，D组中皮质骨和髓腔的正常关系以及骨小梁正常的排列结构重新恢复，B和C组则未见骨髓腔再通。 结论：CPC/bFGF，CPC/VEGF和CPC/bFGF+VEGF 3组复合物均能促进骨组织生长及加速骨缺损的修复；CPC/bFGF+VEGF复合物促进骨组织生长的能力明显优于CPC/bFGF和CPC/VEGF复合物，说明bFGF与VEGF促进骨生长具有协同作用。

Experimental study of calcium phosphate cement mixed with bFGF and VEGF to repair bone defects in rabbit radius

ObjectiveTo explore the synergistic effect of basic fibroblast growth factor (bFGF) and vascular endothelial growth factor (VEGF) on the growth of bone tissue. MethodsA total of 36 rabbits were randomly divided into 4 groups with 9 rabbits (18 sides of the anterior limb) in each group,including group A,B,C, and D.For all rabbits 1.0 cm bone defects was created in both sides of the radius.These bone defect regions were implanted with corresponding composites:group A with calcium phosphate cement (CPC) only,group B with CPC/bFGF,group C with CPC/VEGF while group D with both bFGF and VEGF.At the 3rd,6th, and 12th week after the operation, 6 specimens from each group were randomly selected. The effects were partly assessed by X-ray film examination,bone mineral density (BMD) measurements, biomechanical test and histological observation.ResultsX-ray showed that at the 12th week the bone defects in group D were completely repaired with CPC generally degraded,whereas bone defects in group B and C were only basically repaired. BMD measurements showed that at the 12th week the BMD of group D was significantly higher than that of group B and C (P＜0.05).Biomechanical testing(at the 12th week) showed that the maximum bending load of group D was significantly higher than that of group B and C(P＜0.05).Histological observation indicated that at the 12th week,woven bone had become mature lamellar bone in group D.At the same time,the normal relation of cortical bone and marrow had resumed,and so had the normal structure of trabecula.However,the recanalization of bone marrow cavity could not be seen in group B and C.ConclusionThese 3 kinds of composite:CPC/bFGF, CPC/VEGF and CPC/ bFGF+VEGF can promote the growth of bone tissue and speed up the repair of bone defects. The composite of CPC/bFGF+VEGF is better than the other two composites in promoting the growth of bone tissues, indicating that bFGF and VEGF have a synergistic effect on the growth of bone tissue.