蛋白激酶Cγ亚型基因多态性与重性抑郁障碍的关联性

目的 探讨蛋白激酶Cγ亚型(PKCγ)基因多态性rs3745406与重性抑郁障碍及其症状表型的关联性. 方法 用筛查收集2005年5月至2008年3月在山西医科大学第一医院精神卫生科的门诊或住院部的确诊的中国汉族重性抑郁症患者453例,包括首发蕈性抑郁障碍(80.67％)和部分复发患者(19.33％);采用汉密顿抑郁量表(HAMD)评定病例组症状表型;应用聚合酶链式反应技术(PCR)扩增目的DNA片段,对PCR产物直接测序,检测研究对象rs3745406位点的多态性;使用UNPHASED软件进行等位基因,基因型分析、数量性状分析. 结果 (1)研究样本的基因型分布符合Hardy-Weiberg平衡(X~2=1.46,P=0.25).(2)基因型分布和等位基因频率在重性抑郁障碍患者组和对照组之间差异无统计学意义(P>0.05).(3)PKCγ基因rs3745406位点等位基因与汉密顿抑郁量表(HAMD)自杀临床表型存在明显相关性(X~2=4.746,P=0.0360). 结论 PKCγ基因rs3745406多态性与重性抑郁障碍无明显相关,但与重性抑郁障碍的自杀症状存在显著相关性.

Association study between protein kinase C gamma gene polymorphism and major depressive disorder

Objective To explore the genetic association of PKCγgene rs3745406 polymorphism with maior depressive disorder(MDD)and their clinical pbenotypes. Methods A total of 453 Chinese Hall MDD patients with an initial episode were recruited from our hospital.The symptonmtic phenotypes of these cases were evaluated by HAMD.Polymerase chain reaction (PCR) and difeet sequencing analysis were used to detect the genotype of rs3745406.Associations of allele.genotype and quantitative character were analyzed using the UNPHASED software. Results (1) The distributions of genotypes in both study and control samples were in a Hardy-Weinberg equilibrium(X~2=1.46,P=0.25);(2)No significant allelic and genotypie association Was found in these samples(P>0.05);(3)Further analyses revealed a significant association between rs3745406 locus and HAMD suicidal phenotypes(X~2=4.746,P=0.0360). Conclusion The PKC,rs3745406 polymorphism is not significantly associated with MDD whereas it has a marked association with suicidal symptoms in MDD.