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| 补体C3mRNA在大鼠脑出血模型中的表达及眼镜蛇毒因子对其表达的影响 | |
| 引用本文： | 郑晓梅,刘亮,李小刚,高毅滨,袁云华.补体C3mRNA在大鼠脑出血模型中的表达及眼镜蛇毒因子对其表达的影响[J].中国全科医学,2011,14(24). |
| 作者姓名： | 郑晓梅 刘亮 李小刚 高毅滨 袁云华 |
| 作者单位： | 1. 泸州市人民医院神经内科,四川省泸州市,646000 2. 泸州医学院附属医院神经外科 3. 泸州医学院附属医院神经内科 |
| 摘 要： | 目的 探讨补体C3在实验性大鼠脑出血(CH)急性期的作用机制,研究补体C3在CH后脑水肿中的作用以及应用眼镜蛇毒因子(CVF)干预后对血肿周围组织C3mRNA表达及脑组织含水量的影响.方法 采用立体定向技术,将自体不凝血注入大鼠尾状核制备CH 模型,将雌雄各半SD大鼠分为假手术组、常规CH组和CVF干预组,于制模后3 h、6 h、12 h、1 d、3 d、5 d 和7 d 7个时间点处死动物,用干湿重法测量脑含水量(BWC); RT-PCR法检测补体C3mRNA的表达水平.结果 CH后C3mRNA表达从6 h开始出现增强,3 d时达高峰,血肿周围脑组织含水量在CH后3 h开始增加,3 d时达高峰(P＜0.05),此后逐渐回落,7 d时基本恢复正常水平,脑组织含水量与C3mRNA的表达呈正相关(r=0.758,P＜0.05);经CVF干预后,血肿周围组织C3mRNA表达明显下降,干预组与常规CH组之间比较差异有统计学意义(P＜0.05).CVF干预组脑组织含水量明显低于常规CH组(P＜0.05).结论 脑出血后补体系统激活,C3mRNA表达明显增加,通过CVF干预后,C3mRNA表达下降,脑水肿减轻,能达到神经保护作用. |
| 关 键 词： | 脑出血 补体C3 脑含水量 眼镜蛇毒因子 |
Expression of C3mRNA in a Rat Model of Acute Experimental Intracerebral Hemorrhage and the Impact of Cobra Venom Factor |
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| ZHENG Xiao-mei,LIU Liang,LI Xiao-gang,et al..Expression of C3mRNA in a Rat Model of Acute Experimental Intracerebral Hemorrhage and the Impact of Cobra Venom Factor[J].Chinese General Practice,2011,14(24). | |
| Authors: | ZHENG Xiao-mei LIU Liang LI Xiao-gang |
| Institution: | ZHENG Xiao-mei,LIU Liang,LI Xiao-gang,et al.Department of Neurology,People's Hospital of Luzhou,Luzhou 646000,China |
| Abstract: | Objective To investigate the mechanisims of complement C3 during the acute phase of intracerebral hemorrhage(CH) in rats and its role in the increasing of brain water content;to investigate the effects of cobra venom factor(CVF) on C3mRNA expression and brain water content after intracerebral hemorrhage in rats.Methods Rat CH model was established by injecting autologous blood into the caudate nucleus of rats stereotaxically.SD rats were divided into three groups(hemorrhagic group,sham operation group and C... |
| Keywords: | Cerebral hemorrhage Complement C3 Brain water content Cobra venom factor |
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