膜联蛋白A10在人食管鳞癌及其癌旁组织中的表达及临床意义

目的:探讨膜联蛋白A10(annexin A10)在人食管鳞癌和癌旁组织中的表达以及临床意义.方法:应用半定量RT-PCR、定量PCR、Western blot法,检测 40 对配对人食管鳞癌和癌旁组织中 annexin A10表达差异;并分析该表达差异与临床病理特征如性别、年龄、肿瘤大小、浸润深度、临床分期、淋巴结转移和细胞分化的关系.结果:40 对配对人食管鳞癌组织中,armexinA10 mRNA表达水平癌组织77.06±37.11,癌旁组织66.99±17.62;蛋白表达水平癌组织为1.73±1.30,癌旁组织1.26±1.18,癌组织和配对癌旁组织表达差异有统计学意义(P<0.05).annexin A10在癌组织和癌旁组织的表达差异在淋巴结转移组和未转移组,在高中低分化组均有显著性差异,而与性别、年龄、肿瘤大小、浸润深度、临床分期关系无统计学意义.结论:annexin A10在人食管鳞癌中表达上调.并随肿瘤分化程度降低、淋巴结转移而表达增高.annexin A10可能成为食管鳞癌诊断和预后的一个新的指示靶点.

Detection of annexin A10 in human esophageal squamous cancer tissues

Objective:To investigate the expression of annexin A10(ANXA10) in human esophageal squamous cancer tissues,and to discuss its value in the development of human esophageal squamous cell cancer(SCC). Methods:The expression of annexin A10 in 40 matched-pairs of SCC tissues were detected by RT-PCR,Real-time PCR,Western blot methods. Results:The relative intensities of annexin A10 mRNA expression in SCC tissues and peri-carcinoma tissues were 77.06 ± 37.11,66.99 ± 17.62 respectively. The relative intensities of annexin A10 protein expression in SCC tissues and peri-carcinoma tissues were 1.73 ± 1.30,1.26 ± 1.18 respectively. There were remarkable differences between SCC and esophageal peri-carcinoma tissues. There were also remarkable differences between lymph node metastasis group and non-metastasis group; among well,moderately and poorly differentiated groups. Conclusion:The expression of annexin A10 in SCC is up-regulated and associated with cell differentiation and lymph-node metastasis. Detection of annexin A10 might be a valuable tumor marker and prognostic factor for SCC.