壳聚糖/亚磷酸壳聚糖海绵复合人脐带间充质干细胞构建组织工程骨修复骨缺损的实验研究

目的:探索壳聚糖/亚磷酸壳聚糖海绵复合人脐带间充质干细胞(human umbilical cord mesenchymal stem cells,HUMSCs)构建的组织工程骨植入动物骨缺损处的成骨能力?方法:体外构建组织工程骨;健康新西兰大白兔构造骨缺损模型兔后随机分为3组,即A:组织工程骨植入组;B:支架材料植入组;C:空白对照组,于术后4?8?12 周取骨缺损标本,进行大体标本?影像学?组织学观察成骨情况?结果:术后12周,3种检测手段均发现A组新生骨已经完全将骨缺损填充,并有部分皮质骨将骨缺损两端连接?B组虽有部分骨痂形成,但无连续骨质?C组术后仅有少量骨痂形成?A组成骨能力明显强于B?C组?结论:本实验构建的组织工程骨能修复大段的骨缺损,具有良好的成骨能力,可以作为骨移植的替代材料?

Experimental atudy on repairing bone defects using tissue engineered bone of chitosan/phosphonic chitosan sponge combined with human umbilical cord mesenchymal stem cells

Objective:To investigate the effect of tissue engineered bone of the chitosan/phosphonic chitosan sponge combined with human umbilical cord mesenchymal stem cells (HUMSCs) on repairing bone defects. Methods:First,The tissue engineered bone was constructed in vitro;Second,New Zealand rabbit model with bone defect were divided into groups A,B,and C. The complex sponge was implanted into bone defects in group A;cell scaffold composites were implanted in group B;and group C was as control. The defective bone specimen was collected at 4th,8th,12th week after implantation sugery. The gross and histological changes and the imaging of bone specimen were observed. Results:After 12 weeks,new bone have completely filled the bone defects in group A. Moreover,bone stump have been connected by some cortical bone. While part of porosis can be found in group B,but no continuous sclerotin was observed. Only little porosis can be found in group C. Conclusion:The tissue engineered bone by combining the HUMSCs with chitosan/phosphonic chitosan sponge has the potential of osteogenic ability in rabbit,and can become material substitution for bone transplantation.