| Survivin在胃癌SGC7901顺铂耐药中的作用 |
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| 引用本文： | 董汉章,杨 力,夏建国,陈国玉.Survivin在胃癌SGC7901顺铂耐药中的作用[J].南京医科大学学报,2007,27(9):927-930. |
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| 作者姓名： | 董汉章 杨 力 夏建国 陈国玉 |
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| 作者单位： | 南京医科大学第一附属医院普外科,江苏南京210029 |
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| 摘 要： | 目的:探讨Survivin在小剂量顺铂长期作用于SGC7901细胞导致细胞耐药中的作用机制.方法:体外诱导建立人胃癌顺铂耐药细胞株SGC7901/CDDP,显微镜下观察SGC7901/CDDP与正常SGC7901细胞株的形态差异,应用RT-PCR技术及Western blot技术分别检测SGC7901/CDDP与正常SGC7901细胞株中Survivin mRNA的表达和 Survivin蛋白的表达.结果:SGC7901/CDDP与正常SGC7901细胞株有明显的形态差异;SGC7901/CDDP 中Survivin mRNA的表达和Survivin蛋白的表达都高于SGC7901细胞.结论:SGC7901对顺铂耐药可能与顺铂诱导SGC7901/CDDP 中Survivin表达增加有关.
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| 关 键 词： | 胃癌 耐药 顺铂 Survivin Survivin 人胃癌 顺铂耐药 作用 cisplatin resistance 顺铂诱导 结果 蛋白 表达 mRNA 检测 Western blot 应用 形态差异 耐药细胞株 镜下观察 CDDP 体外诱导 |
| 文章编号： | 1007-4368（2007）09-0927-04 |
| 收稿时间： | 2007/4/19 0:00:00 |
| 修稿时间： | 2007-04-19 |
The role of Survivin in SGC7901 resistance to cisplatin |
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| DONG Hang-zhang,YANG Li,XIA Jian-guo and CHEN Guo-yu.The role of Survivin in SGC7901 resistance to cisplatin[J].Acta Universitatis Medicinalis Nanjing,2007,27(9):927-930. |
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| Authors: | DONG Hang-zhang YANG Li XIA Jian-guo and CHEN Guo-yu |
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| Institution: | Department of General Surgery,the First Affiliated Hospital of NJMU,Nanjing 210029,China |
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| Abstract: | Objective:To study the mechanism on SGC7901 resistance to the low dose cisplatin. Methods:SGC7901/CDDP was established in vitro. The morphology change between SGC7901/CDDP and SGC7901 was observed by microscope. The mRNA and protein expression of Survivin in SGC7901/CDDP and SGC7901 were detected by RT-PCR and Western-blot,respectively. Results:There were morphological differences between SGC7901 and SGC7901/CDDP. The mRNA and protein expressions of Survivin in SGC7901/CDDP were both higher than in SGC7901. Conclusion:SGC7901 resistance to cisplatin may have association with the increased expression level of Survivin in SGC7901/CDDP. |
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| Keywords: | gastric carcinoma drug resistance cisplatin Survivin |
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