温中消痰方对白细胞介素8在S180荷瘤小鼠肿瘤组织表达的影响

目的:观察温中消痰方对寒邪干预S180荷瘤鼠白细胞介素8(interleukin-8,IL-8)及IL-8 mRNA的调节作用,并探讨其对S180荷瘤鼠肿瘤的作用机制。方法:50只小鼠,随机分为荷瘤模型组、寒模组、温中消痰方组、消痰散结方组和化疗组,除模型组外,各组接种肿瘤前1周灌饲0～4℃冰蒸馏水10ml/kg,1次/d,连续3周;第2周瘤细胞用生理盐水稀释至2×107/ml后,注射于小鼠右侧腋下,接种48h后各实验组灌胃给药。寒邪干预组开始给药后上午继续寒邪干预,下午给药;寒模组下午给予生理盐水;各给药组分别给予温中消痰方、消痰散结方及喃氟啶。观察寒邪干预后荷瘤鼠形态学表现及肛温的变化,第22天取瘤测瘤质量和肿瘤体积,计算瘤质量抑瘤率,采用双抗体夹心亲和素-生物素系统-酶联免疫吸附试验(avidin-biotin system-enzyme-labeled immunosorbent assay,ABC-ELISA)法测定S180荷瘤鼠肿瘤组织中IL-8的含量,应用实时聚合酶链式反应(real-time polymerasechain reaction,real-time PCR)法检测肿瘤组织IL-8mRNA的表达。结果:肿瘤组织IL-8的含量,模型组明显高于各实验组(P<0.01);寒模组与温中消痰方组和消痰散结方组相比,差异有统计学意义(P<0.01,P<0.05);温中消痰方组和消痰散结方组IL-8的cDNA扩增条带面积灰度值较寒模组和模型组明显下降(P<0.05)。结论:温中消痰方能抑制S180荷瘤鼠肿瘤的生长,其作用机制可能与降低肿瘤组织IL-8 mRNA的表达,下调S180荷瘤鼠肿瘤组织IL-8的含量有关。

Effects of warming spleen and stomach for dispersing phlegm recipe on expression of interleukin-8 in S180 tumor tissue in mice

OBJECTIVE: To observe the effects of warming spleen and stomach for dispersing phlegm recipe (Wenzhong Xiaotan Recipe, WZXTR), a compound traditional Chinese herbal medicine, on expression of interleukin-8 (IL-8) in S(180) tumor tissue in mice, and to explore the effectiveness and its mechanism. METHODS: Fifty mice were randomly divided into five groups: untreated group, cold water-treated group, cold water and WZXTR-treated group, cold water and dispersing phlegm and eliminating stagnation recipe (Xiaotan Sanjie Recipe, XTSJR, another compound traditional Chinese herbal medicine) treated group, cold water and tegafur-treated group. Mice in the latter 4 groups were fed 0-4 degrees centigrade cold distilled water 10 ml/(kg x d) one week before tumor inoculation for 3 weeks. Mice in the 5 groups were inoculated neoplastic cells which were diluted 2 x 10(7)/ml with normal saline in the right armpit at the second week. Forty-eight hours later, the mice were given drugs intragastrically (WZXTR, XTSJR and tegafur, respectively). The mice were continually intervened by cold water in the morning and given drugs in the afternoon. Mice in the cold water-treated group were given normal saline, and nothing was given in untreated group. Morphology appearance and changes of rectal temperature were observed, and the tumor weight and volume were measured and inhibitory rate was calculated 22 days after tumor inoculation. IL-8 was detected by avidin-biotin system-enzyme-labeled immunosorbent assay (ABC-ELISA) and real-time reverse polymerase chain reaction (real-time PCR). RESULTS: The content of IL-8 in tumor tissue of the untreated group was significantly higher than that of the other groups (P<0.01), and that of the cold water-treated group was higher than that of the cold water and WZXTR-treated group (P<0.01) and the cold water and XTSJR-treated group (P<0.05), respectively. The gray scale value of cDNA amplification strap was descent more evidently in both the cold water and WZXTR-treated group and the cold water and XTSJR-treated group as compared with that of the cold water-treated group and the untreated group (P<0.05). CONCLUSION: WZXTR can restrain tumor growth, and the mechanism may be that it can degrade the expression of IL-8 in S180 tumor.