红景天苷预处理对大鼠全脑缺血再灌注后神经行为学的影响

目的：探讨红景天苷预处理对大鼠全脑缺血再灌注后神经行为学的影响及其可能机制。 方法：60只SD雄性大鼠随机分为假手术组、模型组及红景天苷预处理组，每组20只。采用四血管阻断法建立全脑缺血再灌注模型。红景天苷预处理组大鼠予腹腔注射红景天苷12mg／（kg·d），连续7d，最后一次给药后30min建立急性全脑缺血再灌注模型。再灌注后24h每组各取5只大鼠，取右侧大脑采用干湿重法计算脑组织含水量；左侧大脑取海马测定超氧化物歧化酶（superoxide dismutase，SOD）活性及丙二醛（malondialdehyde，MDA）含量。每组其余15只大鼠分别于再灌注前及再灌注后6、12、24、48和96h进行神经损害严重程度评分（neurological severity score，NSS）；并于再灌注后第5天开始进行Morris水迷宫实验。 结果：模型组脑组织含水量、SOD活性、MDA含量、NSS、平均潜伏期及探索实验中在第2象限时间比与假手术组比较差异有统计学意义（P〈0．05）；红景天苷预处理组与模型组比较，脑含水量降低，SOD活性增高，MDA含量降低，NSS降低，平均潜伏期缩短，第2象限时间比增高，差异有统计学意义（P〈0．05）。 结论：红景天苷可减轻大鼠全脑缺血再灌注后脑水肿程度，缓解海马区自由基代谢异常，改善认知功能。

Effects of salidroside-pretreatment on neuroethology of rats after global cerebral ischemia-reperfusion

Objective： To study the effects of salidroside-pretreatment on changes of neuroethology in rats with global cerebral ischemia-reperfusion injury so as to investigate its probable mechanism.
Methods： Sixty SD male rats were randomly divided into sham-operated group, untreated group and salidroside-pretreated group. The rats in salidroside-pretreated group were intraperitoneally administered with salidroside for seven days. The dose of salidroside was 12 mg/（kg·d）. Thirty minutes after the last administration, the acute global cerebral ischemia-reperfusion in rats of the untreated group and the salidroside-pretreated group was induced by using the modified Pulsinelli＇s 4-vessel occlusion method. Five rats in each group were killed to obtain their brains 24 hours after reperfusion. The water content in the right brain was measured by calculating the ratio of dry weight to wet weight of the right brain. Activity of superoxide dismutase （SOD） and content of malondialdehyde （MDA） in hippocampus of the rats were measured. Then neurological severity scores （NSSs） of the other 15 rats in each group were observed respectively before and 6, 12, 24, 48 and 96 h after reperfusion. At the fifth day after reperfusion, the test of Morris water maze was carried out to examine the memories and learning abilities of the rats.
Results： The content of MDA, the activity of SOD, the NSS, the mean incubation period and the ratio of time in the second quadrant in the untreated group were significant different from those in the sham-operated group （P〈0.05）. Compared with the untreated group, the brain water content, the content of MDA and the NSS degraded, and the mean incubation period shortened in salidroside-pretreated group. The activity of SOD and the ratio of residence time in the second quadrant increased in salidroside-pretreated group as compared with the untreated group （P〈0. 05）.
Conclusion： Salidroside can reduce the degree of cerebral edema of rats with global cerebral ischemiareperfusion injury, relieve the metabolism abnormity of free radical and improve the function of cognition.