金龙蛇颗粒对裸鼠原位移植人胃癌MK-45细胞凋亡的影响

目的:评价金龙蛇颗粒对裸鼠原位移植人胃癌MKN-45细胞的抑制作用。方法:50只裸鼠建立原位移植人胃癌MKN-45细胞模型,随机分为模型组,金龙蛇颗粒高、中、低剂量组和5-FU干预组。各组裸鼠予以相应药物实施干预。观察各组荷瘤裸鼠一般情况,肿瘤生长情况及抑瘤率。流式细胞仪检测肿瘤细胞周期分布及细胞凋亡情况。采用膜联蛋白V-异硫氰酸荧光素/碘化丙啶(Annexin V-fluoresceinisothiocyanate/propidium iodide,Annexin V-FITC/PI)双标记染色法鉴别早期凋亡细胞、晚期凋亡细胞和坏死细胞。电镜下观察肿瘤细胞的超微结构变化。结果:金龙蛇颗粒高、中、低剂量组的抑瘤率分别为68.13%、55.94%和50.31%,呈剂量依赖效应,与5-FU干预组抑瘤率比较,差异无统计学意义。金龙蛇颗粒各剂量组肿瘤细胞凋亡率为22.81%~38.54%,亦呈剂量依赖效应,且肿瘤细胞主要被阻滞于G0/G1期。AnnexinV-FITC/PI双标记染色结果显示,金龙蛇颗粒各剂量组以早期凋亡细胞居多,5-FU干预组则以晚期凋亡细胞和坏死细胞居多。结论:金龙蛇颗粒对裸鼠原位移植人胃癌MKN-45细胞具有较好的抑制作用,促进MKN-45细胞凋亡可能是其抗肿瘤治疗的主要作用机制之一。

Effects of Jinlongshe Granules on apoptosis of MKN-45 human gastric cancer cells orthotopically transplanted in nude mice

Objective: To assess the effects of Jinlongshe Granules(JLSG) on tumor growth of gastric(carcinoma.) Methods: Fifty nude mice orthotopically transplanted with MKN-45 human gastric cancer cells were divided into five groups: untreated group,5-fluorouracil(5-FU)-treated group and high-,medium-,and low-dose JLSG-treated groups.Corresponding antitumor drugs were administered in each group except the untreated group.The antitumor effects in vivo were evaluated.Cell cycle distribution and apoptosis of MKN-45 human gastric cancer cells were determined by using flow cytometry(FCM) and Annexin V-fluorescein isothiocyanate/propidium iodide(Annexin V-FITC/PI) staining assay.The ultrastructure of MKN-45 gastric(cancer) cells was observed by transmission electron microscope.Results: In the mice treated with high-,medium-,and low-dose JLSG,the growth inhibition rates of gastric cancer were 68.13%,55.94% and 50.31% respectively,and this antitumor effect was dose-dependent.In the mice treated with intraperitoneal injection of(5-FU,) the growth inhibition rate of gastric cancer was 53.43% and not much different from those treated with JLSG.The apoptotic rates in the high-,medium-,and low-dose JLSG-treated groups were 22.81%,(28.27%) and 38.54% respectively,in a dose-dependent manner,with the cell cycle arrested at G_(0)/G_(1) phase.An Annexin V-FITC/PI staining assay revealed that the percentages of early apoptotic cells in the three dose JLSG-treated groups were all significantly higher than that in the 5-FU-treated group,whereas the late apoptotic and necrotic cells were much more in the 5-FU-treated group than those in the three dose JLSG-treated groups.Conclusion: Jinlongshe Granules exert an inhibiting effect on MKN-45 human gastric cancer cells orthotopically transplanted in nude mice by promoting the cell apoptosis.