| LuxS/AI-2对伤寒沙门菌基因表达的调节 |
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| 引用本文： | 罗哲,王敏,杜鸿,王菲,孟彦辰,倪斌,徐顺高,黄新祥.LuxS/AI-2对伤寒沙门菌基因表达的调节[J].江苏大学学报(医学版),2011,21(1):34-41. |
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| 作者姓名： | 罗哲 王敏 杜鸿 王菲 孟彦辰 倪斌 徐顺高 黄新祥 |
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| 作者单位： | 1. 江苏大学生命科学研究院,江苏,镇江212013
2. 江苏大学基础医学与医学技术学院,江苏,镇江212013 |
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| 基金项目： | 国家自然科学基金资助项目 |
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| 摘 要： | 目的:探讨伤寒沙门菌luxS基因在葡萄糖存在下对细菌对数生长中期基因表达调控的影响.方法:应用自杀质粒介导的同源重组方法制备伤寒沙门菌luxS基因缺陷变异株;比较野生株与缺陷株的生长情况及动力差异;用哈氏弧菌BB170作为报告菌株检测不同时期野生株与缺陷株的生物发光;利用伤寒沙门菌全基因组芯片技术,比较在葡萄糖存在下伤...
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| 关 键 词： | 伤寒沙门菌 luxS 群体感应 生物发光 基因芯片 |
Analysis of Genes expression regulation controlled by luxS/AI-2 in Salmonella enterica serovar Typhi |
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| LUO Zhe,WANG Min,DU Hong,WANG Fei,MENG Yan-chen,NI Bin,XU Shun-gao,HUANG Xin-xiang.Analysis of Genes expression regulation controlled by luxS/AI-2 in Salmonella enterica serovar Typhi[J].Journal of Jiangsu University Medicine Edition,2011,21(1):34-41. |
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| Authors: | LUO Zhe WANG Min DU Hong WANG Fei MENG Yan-chen NI Bin XU Shun-gao HUANG Xin-xiang |
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| Institution: | LUO Zhe1,WANG Min2,DU Hong2,WANG Fei2,MENG Yan-chen2,NI Bin2,XU Shun-gao2,HUANG Xin-xiang2 (1.School of Life Science,2.School of Medical Science and Laboratory Medicine,Jiangsu University,Zhenjiang Jiangsu 212013,China) |
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| Abstract: | Objective: To elucidate the influence of LuxS on gene expression regulation of Salmonella enterica serovar Typhi(S.Typhi) at mid-log phase in the presence of glucose.Methods: The luxS deleted mutant of S.Typhi was prepared by the homologous recombination mediated by suicide plasmid;the differences of growth and motility between wild-type(WT) and mutant were compared;luminescence assays were performed in WT and mutant at different growth phases in the presence and absence of glucose with reporter strain Vibr... |
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| Keywords: | Salmonella enterica serovar Typhi luxS quorum sensing luminescence microarray |
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