| SsrB对伤寒沙门菌氧应激早期基因表达的调节 |
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| 引用本文： | 王敏,罗哲,杜鸿,孟彦辰,王菲,倪斌,徐顺高,黄新祥.SsrB对伤寒沙门菌氧应激早期基因表达的调节[J].江苏大学学报(医学版),2011,21(1):29-33,41. |
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| 作者姓名： | 王敏 罗哲 杜鸿 孟彦辰 王菲 倪斌 徐顺高 黄新祥 |
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| 作者单位： | 1. 江苏大学基础医学与医学技术学院生物化学研究室
2. 江苏大学生命科学研究院,江苏,镇江,212013 |
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| 基金项目： | 国家自然科学基金资助项目 |
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| 摘 要： | 目的:研究伤寒沙门菌ssrB基因在氧应激早期对其他基因表达的调节.方法:通过同源重组的方法利用自杀质粒制备伤寒沙门菌ssrB基因缺陷变异株;采用伤寒沙门菌全基因组芯片比较野生株和ssrB基因缺陷变异株在氧应激早期的基因表达差异,并对其中部分表达差异基因进行实时定量PCR验证;利用HeLa细胞进行细菌侵袭实验,研究ssr...
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| 关 键 词： | 伤寒沙门菌 ssrB 氧应激 基因芯片 侵袭 |
Regulation of ssrB on gene expression of Salmonella enterica serovar Typhi at early stage of oxidative stress |
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| WANG Min,LUO Zhe,DU Hong,MENG Yan-chen,WANG Fei,NI Bing,XU Shun-gao,HUANG Xin-xiang.Regulation of ssrB on gene expression of Salmonella enterica serovar Typhi at early stage of oxidative stress[J].Journal of Jiangsu University Medicine Edition,2011,21(1):29-33,41. |
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| Authors: | WANG Min LUO Zhe DU Hong MENG Yan-chen WANG Fei NI Bing XU Shun-gao HUANG Xin-xiang |
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| Institution: | WANG Min1,LUO Zhe2,DU Hong1,MENG Yan-chen1,WANG Fei1,NI Bing1,XU Shun-gao1,HUANG Xin-xiang1(1.Department of Biochemistry,School of Basic Medical Science and Laboratory Medicine,2.School of Life Science,Jiangsu University,Zhenjiang Jiangsu 212013,China) |
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| Abstract: | Objective: To investigate the regulation of ssrB on gene expression of Salmonella enterica serovar Typhi(S.Typhi) at early-stage of oxidative stress.Methods: The ssrB deleted mutant of S.Typhi was generated through homologous recombination mediated by suicide plasmid;the gene expression profiles of the wild-type strain and the ssrB deleted mutant at early-stage of oxidative stress was investigated by genomic microarray assay;qRT-PCR was performed to further validate the results of microarray assay.The HeLa ... |
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| Keywords: | Salmonella enterica serovar Typhi ssrB oxidative stress gene microarray invasion |
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