血管紧张素Ⅱ-2型受体基因A1675 G多态性与原发性高血压的相关性研究

目的:研究血管紧张素Ⅱ-2型受体(AT2R)基因A1675G单核苷酸多态性(SNPs)与中国汉族人群原发性高血压(Essential Hypertension,EH)的关系,探讨AT2R基因在EH发病机制中的作用.方法:应用等位基因特异性引物PCR方法检测181例EH患者(EH组)和187例健康人(对照组)的AT2R基因型,比较各组间的等位基因频率及基因型分布差异,分析AT2R基因A1675G多态性与高血压的关系.结果:AT2R基因A1675G的A等位基因频率在EH组明显高于对照组(53.3%vs44.4%),但两组间差异无显著性意义(P＞0.05).在男性组中,EH组的A等位基因频率明显高于对照组(53.6%vs42.9%,P＞0.05);而在女性组中,EH组和正常对照组间各基因型(AA,AG,GG)频率及等位基因频率分布的差异均无显著性意义(P＞0.05).结论:AT2R的A等位基因频率在EH组的分布较正常对照组高,但无统计学意义,尚不能确定AT2R基因A1675G多态性与中国人原发性高血压的发生存在相关性.

Relationship Between A1675G Polymorphism of Angiotensin Ⅱ type 2 - receptor Gene and Essential Hypertension

Objective: To explore the role of the single nucleotide polymorphisms (SNPs) at position A1675G of angiotensin II type 2 -receptor (AT2R) in patients with essential hypertension (EH) in the Chinese Han nationality population . Methods: Genotypes of the polymorphisms of AT2R gene in 181 patients with EH (EH group) and 187 healthy volunteers (control group) were determined by the allele - specific primer polymerase chain reaction (PCR). We analyzed genotype distribution and allele frequency of AT2R gene between EH group and control group were compared so as to evaluate the contribution of polymorphism of AT2R gene to hypertension, re-suits ; There was a significant increase in allele frequency of A167S in EH group compared with control group (53. 3 % vs 44.4 % ) , but the difference in two groups was of no statistical significance (P > 0.05). This study showed a significant increase in allele frequency of A1675 (53.6% vs 42.9% , P > 0. 05) in male essential hypertensive patients. There were no differences in genotypes ( AA, AG, GG) distribution and allele frequency of AT2R A1675G between female EH group and control group (P>0.05). conclusion; The frequency of A allele in essential hypertension is higher than that in control group, but there is no statistical significance. The relationship between AT2R gene A1675G polymorphism and essential hypertensive patients of the Chinese Han nationality population.