| 利用基因芯片技术对小鼠隐睾相关基因谱研究 |
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| 引用本文： | 杨波,袁建林,高晓康,秦卫军,刘飞,王禾,邵晨,刘贺亮,康福霞.利用基因芯片技术对小鼠隐睾相关基因谱研究[J].第四军医大学学报,2009,30(10):897-899. |
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| 作者姓名： | 杨波 袁建林 高晓康 秦卫军 刘飞 王禾 邵晨 刘贺亮 康福霞 |
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| 作者单位： | 杨波,袁建林,秦卫军,刘飞,王禾,邵晨,刘贺亮,康福霞,YANG Bo,YUAN Jian-Lin,QIN Wei-Jun,LIU Fei,WANG He,SHAO Chen,LIU He-Liang,KANG Fu-Xia(第四军医大学西京医院泌尿外科,陕西,西安,710033);高晓康,GAO Xiao-Kang(兰州军区乌鲁木齐总医院泌尿外科,新疆,乌鲁木齐,830000)
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| 摘 要： | 目的：利用基因芯片研究小鼠隐睾相关基因谱的改变.方法：建立小鼠隐睾模型,分别提取正常及异常睾丸组织的mRNA,制备杂交用cDNA探针,在包含小鼠4000条cDNA片段的基因芯片上点样、杂交、洗涤后,通过计算机观察二者表达谱的差异情况,并对其中表达下降的COX8a进行RNA斑点印迹杂交以验证基因芯片结果.结果：通过基因芯片筛选,获得34条与小鼠隐睾相关的基因.RNA斑点印迹支持基因芯片杂交结果.结论：利用基因芯片研究小鼠隐睾睾丸组织与正常睾丸组织差异表达的基因谱可以用于高通量筛选隐睾相关基因以及进一步的研究;COX8a可能在隐睾症的发生与进展过程中起一定的作用.
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| 关 键 词： | 基因芯片 隐睾 差异表达 |
Investigation of differential gene expression profiles in mice cryptorchid testes by cDNA microarray |
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| YANG Bo,YUAN Jian-Lin,GAO Xiao-Kang,QIN Wei-Jun,LIU Fei,WANG He,SHAO Chen,LIU He-Liang,KANG Fu-Xia.Investigation of differential gene expression profiles in mice cryptorchid testes by cDNA microarray[J].Journal of the Fourth Military Medical University,2009,30(10):897-899. |
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| Authors: | YANG Bo YUAN Jian-Lin GAO Xiao-Kang QIN Wei-Jun LIU Fei WANG He SHAO Chen LIU He-Liang KANG Fu-Xia |
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| Institution: | YANG Bo1,YUAN Jian-Lin1,GAO Xiao-Kang2,QIN Wei-Jun1,LIU Fei1,WANG He1,SHAO Chen1,LIU He-Liang1,KANG Fu-Xia11Department of Urology,Xijing Hospital,Fourth Military Medical University,Xi'an 710033,China,2Department of Urology,Wulumuqi General Hospital,Lanzhou Military Area Command,Wulumuqi 830000 |
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| Abstract: | AIM:To study the differential gene expression profiles between the normal and cryptorchid mice testes by cDNA microarray. METHODS: BLAC mice were unilaterally produced cryptorchidism artificially. Four weeks later, we extracted mRNA from both normal and cryptorchid testes, produced cDNA probes and employed them on cDNA microarray to detect the differential gene expression profiles. RNA dot blotting was taken on COX8a, a down-expressed gene in the previous hybridization, to validate the microarrays. RESULTS:... |
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| Keywords: | cDNA microarray cryptorchidism differential expression |
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