蛋白酶激活受体-2激动剂对人皮肤和扁桃体肥大细胞释放组胺和类胰蛋白酶的影响

目的:研究蛋白酶激活受体-2(PAR-2)激动剂对人皮肤和扁桃体肥大细胞释放组胺和类胰蛋白酶的影响.方法:用PAR-2激动剂激发经酶悬浮的人皮肤和扁桃体肥大细胞,并分别测量类胰蛋白酶和组胺的释放量.结果:PAR-2激动剂丝-亮-异亮-甘-赖-缬(SLIGKV)可引起皮肤肥大细胞组胺释放,但对类胰蛋白酶释放无影响.相反,SLIGKV可引起扁桃体肥大细胞的类胰蛋白酶,而不是组胺释放.另外一种PAR-2激动剂反肉桂酰-亮-异亮-甘-精-亮-鸟-酰胺](te-LIG-RLO-NH2)比SLIGKV-NH2诱导类胰蛋白酶和组胺释放的作用弱.结论:我们首次报道了皮肤和扁桃体肥大细胞具有在体外释放类胰蛋白酶的特性.皮肤和扁桃体肥大细胞释放类胰蛋白酶和组胺的差别揭示了一种新的肥大细胞对刺激产生反应的异质性的类型,提示人类肥大细胞至少有2种脱颗粒信号的自我放大机制.

Effect of proteinase activated receptor2 agonists on histamine and tryptase release from human skin and tonsil mast cells

AIM: To investigate the effect of proteinase activated receptor-2 (PAR-2) agonists on histamine and tryptase release from human skin and tonsil mast cells. METHODS: Enzymatically dispersed mast cells from human skin and tonsil were challenged with PAR-2 stimuli and tryptase and histamine release were determined. RESULTS: PAR-2 agonist peptide SLIGKV induced the release of histamine, but not that of tryptase from skin mast cells. However, it induced the release of tryptase, but not that of histamine from tonsil mast cells. Another PAR-2 agonist peptide tc-LIGRLO appeared less potent than SLIGKV in induction of release of histamine and tryptase. CONCLUSION: We are the first to report the in vitro tryptase release properties of human skin and tonsil mast cells. The differences in release of tryptase and histamine from skin and tonsil mast cells suggest a novel type of mast cell heterogeneity in response to stimulation, and indicate that there might be at least two self-amplification mechanisms of degranulation in human mast cells.