MCL1在鼻咽癌中的表达水平及临床意义

目的 研究髓样细胞白血病蛋白1（myeloid cell leukemia-1 protein，MCL1）在鼻咽癌组织和鼻咽癌细胞中的表达情况，并分析其表达水平与鼻咽癌患者临床预后指标之间的关系。方法 运用免疫组化方法检测鼻咽癌组织和正常鼻腔黏膜组织中MCL1蛋白的表达差异，并分析MCL1表达与鼻咽癌患者临床病理指标之间的关系；运用Western blot法检测人鼻咽癌细胞系CNE2、CNE1以及人永生化鼻黏膜上皮细胞NP-69中MUC1蛋白表达情况。结果 免疫组化结果显示，鼻咽癌组织中MCL1表达阳性率为58.3%（35/60），在正常鼻腔黏膜组织中MCL1阳性表达率23.8%（5/21），鼻咽癌组织的MCL1表达水平显著高于正常鼻腔黏膜组织（P<0.05）；MCL1的表达水平与鼻咽癌患者的T分级、临床分期及淋巴结转移显著相关（P<0.05），而与患者年龄、性别及分化程度无显著相关性（P>0.05）；同时，MCL1在鼻咽癌细胞系CNE2及CNE1中的表达高于NP69细胞，差异均有统计学意义（P<0.05）；结论 MCL1在鼻咽癌中可能起到促进肿瘤进展的作用，患者的MCL1表达水平可能与其预后相关。

Expression of MCL1 in Human Nasopharyngeal Carcinoma and Its Clinical Significance

Objective To explore the expression of myeloid cell leukemia 1 protein (MCL1) and its clinical significance in nasopharyngeal carcinoma and nasopharyngeal cells. Methods Immunohistochemistry was used to detect the MCL1 expression levels of nasopharyngeal carcinoma and normal nasal mucosal tissues,and to analysis the relationship between MCL1 expression and clinic pathological features of patients with nasopharyngeal carcinoma. Western blot was used to analysis the MCL1 expression levels of nasopharyngeal cells CNE2,CNE1and normal nasal mucosal cell NP-69. Results The positive rate of MCL1 in nasopharyngeal carcinoma patients was 58.3%(35/60), and the positive rate of MCL1 in normai patients was 23.8%(5/21).The results showed the expression level of MCL1 in nasopharyngeal carcinoma was significantly higher than normal tissues(P<0.05). MCL1 expression had obvious correlation with clinical stage, T stage and lymph node metastasis of nasopharyngeal carcinoma(P<0.05). The expression level of MCL1 in CNE2 and CNE1 cells was significantly higher than in NP-69 cell. Conclusion MCL1 is positively expressed in the majority of NPC, and its expression levels may be related to the patient''s prognosis.