| 突触可塑性研究——Ⅰ.整肌胆碱酯酶—银、金联合染色法的建立及意义 |
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| 引用本文： | 袁锦楣,李彤,杨俊华.突触可塑性研究——Ⅰ.整肌胆碱酯酶—银、金联合染色法的建立及意义[J].北京大学学报(医学版),1990(5). |
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| 作者姓名： | 袁锦楣 李彤 杨俊华 |
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| 作者单位： | 北京医科大学第一医院神经病学研究室
(袁锦楣，李彤)，北京医科大学第一医院神经病学研究室(杨俊华) |
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| 摘 要： | 整肌胆碱脂酶-银、金联合染色法可以全面地反映穿过肌肉的全部神经树及运动终板的分布情况,轴索染成棕黑色、终板染成蓝色,对比清晰,轮廓清楚,不仅可测量终板大小,而且可观察终板内神经末梢的形状,并可从一个层面到另一个层面追踪神经末梢的发芽情况,对研究突触可塑性极有价值。
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| 关 键 词： | 突触可塑性 终板 发芽 |
A STUDY ON SYNAPTIC PLASTICITY: A NEW STAIN METHOD THROUGHOUT WHOLE MOUNTS SKELETAL MUSCLES USING COMBINED SILVER, GOLD AND CHOLINESTERASE STAIN |
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| Yuan Jinmei,et al.A STUDY ON SYNAPTIC PLASTICITY: A NEW STAIN METHOD THROUGHOUT WHOLE MOUNTS SKELETAL MUSCLES USING COMBINED SILVER, GOLD AND CHOLINESTERASE STAIN[J].Journal of Peking University:Health Sciences,1990(5). |
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| Authors: | Yuan Jinmei |
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| Institution: | Yuan Jinmei,et al Department of Neurology,The First Hospital |
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| Abstract: | A new stain method throughout whole mounts of skeletal muscles using combined silver, gold andcholinesterase stain was reported, which allowed the visualization of the complete axonal tree, motornerve terminals and the cholinesterase at neuromuscular junctions. This method was rapid, reliable andsimple and allowed to read the NMJs and its sprouting on different levels. Because stained muscleswere mounted whole and need not be sectioned or teased into single fiber, topographical relationshipwas preserved in a way which had not been possible by using other stain methods. |
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| Keywords: | Neuromuscular junction Sprouting |
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