CIK细胞在肿瘤免疫治疗中的作用

目的研究细胞因子诱导的杀伤细胞（CIKcells）的体外扩增特性及其在抗肿瘤免疫治疗中的作用。方法通过提取健康供血者的PBMNC,培养诱导CIK细胞,显微镜下观察其扩增倍数、增殖速率,流式细胞仪分析细胞免疫表型,用MTT法观察其对K562细胞的体外杀伤效应,并通过流式细胞仪进一步分析30例实体瘤患者CIK细胞输注前后外周血免疫活性细胞表型的改变。结果经2周培养,CIK细胞平均增加了30倍,总数达（4.8～5.0）×109,活性细胞比例〉90%;体外杀伤率约50%,表型检测效应细胞CD3＋、CD3＋CD4＋、CD3＋CD8＋、CD3＋CD56＋亚群比例显著升高,差异有高度统计学意义（P〈0.01）。实体瘤患者CIK细胞输注后外周血中免疫活性细胞比例也明显升高,差异也有统计学意义（P〈0.05）。结论CIK细胞在体内外能明显抑制肿瘤生长,有效改善患者的免疫功能,为晚期肿瘤治疗提供了新的方法。

Study on Anti-tumor Effects of CIK Cells in Tumor Immunol Therapy

Objective Study the proliferation and anti-tumor effects of cytokine induced killer cells （CIK cells） in tumor immunol therapy. Methods CIK cells were generated by culturing PBMNC in the presence of IFN-r, IL-2 and anti-CD3 McAb. CIK ultures were analyzed on different time points by FACS. The killing efficacy in tumor cell line（K562） were detected by MTT assay. To treat 30 patients suffered from malignant tumors with CIK cells. The function of cell immunity in patients peripheral blood were measured by immunofluorescence before and after 2 weeks of treatment respectively. Results After 2 weeks of culture, CIK cells expanded about 30 folds. The percentage of CD3＋,CD3＋CD4＋,CD3＋,CD8＋,CD3＋CD56＋ cells increased significantly after cytokines induction （P〈0.01）.CIK cells had great cytotoxity against K562 cell line in vitro ,the median inhibitory rate was 50%. The percentages of CD3＋, CD3＋CD4＋, CD3＋CD8＋, CD3＋CD56＋ of post-treatment patients changed significantly compared with that in the no-treatment group （P〈0.05）. Conclusion CIK cells have a stronger significsnt suppression effect on growth of K562 cell line in vitro. It also could improve immune function of patients .The results all provide an basis for CIK cells in clinical application as a adaptive immunotherapy.