| Effects of Oxymatrine on the Apoptosis of Human Esophageal Carcinoma Eca109 Cell Line and Its Mechanism |
| |
| 作者姓名： | 靳毅 胡建莉 王琼 李振宇 陈叶珊 |
| |
| 作者单位： | Department of Clinical Laboratary Union Hospital，Tongji Medical College，Huazhong University of Science and Technology，Center of Oncology，Union Hospital，Tongji Medical College，Huazhong University of Science and Technology，Center of Oncology，Union Hospital，Tongji Medical College，Huazhong University of Science and Technology，Center of Oncology，Union Hospital，Tongji Medical College，Huazhong University of Science and Technology，Center of Oncology，Union Hospital，Tongji Medical College，Huazhong University of Science and Technology，Wuhan 430022，China，Wuhan 430022，China，Wuhan 430022，China，Wuhan 430022，China，Wuhan 430022，China |
| |
| 摘 要： | The effects of Oxymatrine （Oxy） on the proliferation and apoptosis of human esophageal carcinoma Ecal09 cell line and the mechanism were investigated. The human esophageal carcinoma Eca 109 celis were cultured in vitro. The Oxy-induced apoptosis of Eca 109 cells was assayed by using flow cytometry. The expressions of p-ERKII2, Cyclin D1, p21^waf/cipl, Bax and Bcl-2 were detected by Western blot. Flow cytometry revealed that Oxy could induce the apoptosis of Eca l09 cells. Western blot showed that Oxy of different concentrations suppressed the expressions of p-ERK1/2, Cyclin D1 and Bcl-2, but up-regulated the expression of p21waf/cip1 and Bax, and the ratio of Bax/Bcl-2 was increased. It was suggested the Oxy could induce the apoptosis of Eca l09 cells, which might be related to the upregulation of p21waf/cip1 and the downregulation of p-ERK1/2, Cyclin D1 and p21^waf/cip1. The possible pathway may be related to Bcl-2/Bax.
|
| 关 键 词： | 氧化苦参碱 食道癌 细胞外信号调节激酶 细胞凋亡 |
Effects of Oxymatrine on the apoptosis of human esophageal carcinoma Eca109 cell line and its mechanism |
| |
| Yi?Jin,Jianli?Hu,Qiong?Wang,Zhenyu?Li,Yeshan?Chen.Effects of Oxymatrine on the Apoptosis of Human Esophageal Carcinoma Eca109 Cell Line and Its Mechanism[J].Journal of Zuazhong University of Science and Technology: Medical Edition,2008,28(3):314-316. |
| |
| Authors: | Yi Jin Jianli Hu Qiong Wang Zhenyu Li Yeshan Chen |
| |
| Institution: | 1. Department of Clinical Laboratary, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, 430022, China
2. Center of oncology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, 430022, China |
| |
| Abstract: | The effects of Oxymatrine(Oxy) on the proliferation and apoptosis of human esophageal carcinoma Eca109 cell line and the mechanism were investigated.The human esophageal carcinoma Eca109 cells were cultured in vitro.The Oxyinduced apoptosis of Eca109 cells was assayed by using flow cytometry.The expressions of p-ERK1/2,Cyclin D1,p21waf/cip1,Bax and Bcl-2 were detected by Western blot.Flow cytometry revealed that Oxy could induce the apoptosis of Eca109 cells.Western blot showed that Oxy of different concentrations suppressed the expressions of p-ERK1/2,Cyclin D1 and Bcl-2,but up-regulated the expression of p21waf/cip1 and Bax,and the ratio of Bax/Bcl-2 was increased.It was suggested the Oxy could induce the apoptosis of Eca109 cells,which might be related to the upregulation of p21waf/cip1 and the downregulation of p-ERK1/2,Cyclin D1 and p21waf/cip1.The possible pathway may be related to Bcl-2/Bax. |
| |
| Keywords: | Oxymatrine esophageal carcinoma extracellular signal-regulated kinase apoptosis |
| 本文献已被 CNKI 维普 万方数据 SpringerLink 等数据库收录！ |
|
