纳米羟基磷灰石／聚酰胺66／富血小板血浆复合物修复兔股骨中段骨缺损的实验研究

目的：探讨纳米羟基磷灰石／聚酰胺66／富血小板血浆（n-HA／PA66／PRP）复合物修复兔股骨中段骨缺损的疗效。方法新西兰大白兔共40只，切除左股骨中段1 cm 连同骨膜的骨质造成骨缺损模型后随机分为两组，实验组植入 n-HA／PA66／PRP 复合物后予以钢板固定；对照组植入 n-HA／PA66后予以钢板固定。术后2、4、8、12周每时间点处死5只兔子，进行 X 线片、大体标本观察、组织学、免疫组织化学染色等观察股骨骨缺损愈合情况。结果术后所有动物无感染、死亡及植入物脱落，大体标本及组织学结果显示术后2周内实验组开始有新生骨组织，随着时间的延长，实验组新骨生长速度和数量明显优于对照组；术后12周 Lane-Sandhu 法 X 线片评分显示实验组（6．80±2．05）分，对照组（4．20±1．30）分，二者比较差异有统计学意义（P ＜0．05）；免疫组织化学染色结果显示实验组血管内皮细胞生长因子表达强度在术后第2、4周时与对照组比较，差异有统计学意义（P ＜0．05），在术后第8、12周时与对照组比较差异无统计学意义（P ＞0．05）。结论n-HA／PA66／PRP 复合物具有促进骨质愈合的作用，尤其在早期修复骨缺损的效果优于 n-HA／PA66。

The experimental research of nano-hydroxyapatite/polyamide 66/platelet-rich plasma compound repair of rabbit femoral bone defect

Objective To investigate the therapeutic effects of nano-hydroxyapatite/polyamide 66/platelet-rich plasma com-pound(n-HA/PA66/PRP)on the recovery of rabbit femur bone defect.Methods 40 New Zealand rabbits were artificially made to be bone defect by resecting the 1 cm substantia ossea with periosteum of femur,and were divided into two groups averagely depen-ding on implanted materials:experimental group(n-HA/PA66/PRP),control group (n-HA/PA66).Every five rabbits were sacri-ficed on week 2,4,8,12,and the femur healing status was observed by X ray,histology,and immunohistochemistry.Results No rabbit was infected or died,no implantation objects dropped.Gross observation,X-ray result and histology results demonstrated that the experimental group began to have a new bone tissue at 2 weeks after the operation,with the extension of time,the experimental group new bone growth speed and the quantity was better than the control group.The Lane-Sandhu method X-ray score showed that the experimental group (6.80±2.05)points and the control group(4.20±1.30)points at 12 weeks after the operation,and there were significant differences between two groups(P <0.05).Immunohistochemistry indicated that expression intensity of Vas-cular endothelial growth factor at 2 weeks and 4 weeks were significant differences between two groups(P <0.05),but there was no significant difference at 8 weeks and 12 weeks (P >0.05).Conclusion PRP combined with n-HA/PA66 artificial bone could accel-erate the healing of bone defect,and the effect of repair of bone defect is better than that of n-HA/PA66 artificial bone in the early.