鼻咽癌患者外周血HLA-DR-CD33+CD14-CD11b+的MDSC检测

目的 探讨鼻咽癌患者外周血HLA-DR-CD33+ CD14-CD11 b+的髓源性抑制细胞(MDSC)的分布比例,为鼻咽癌免疫治疗提供有益指导.方法 选择该院肿瘤科收治的45例鼻咽癌患者和20例健康志愿者作为健康对照组,均在第1次化疗前和第2周期诱导化疗后7d抽取患者外周血标本,同时抽取健康对照组人群外周血;并在流式细胞仪上做HLA-DR-CD33+CD14-CD1 1b+的MDSC测定.结果 鼻咽癌患者外周血存在MDSC比例明显高于健康对照组(P＜0.05).而且随着Ⅱ、Ⅲ、Ⅳ期分期的升高,鼻咽癌患者外周血MDSC比例也逐渐升高(P＜o.05)..鼻咽角化癌、鼻咽分化型非角化癌和未分化型非危化癌3种不同病理类型鼻咽癌患者外周血MDSC比例均高于健康对照组(P＜0.05).3种不同病理类型的鼻咽癌患者外周血MDSC比例也差异有统计学意义(P＜0.05).经过2周期诱导化疗后各期鼻咽癌的患者外周血MDSC比例均有显著降低(P＜0.05),但其比例水平仍然高于健康对照组人群.结论 鼻咽癌患者外周血HLA-DR-CD33+ CD14-CD1 1b+的MDSC比例明显升高,提示可能与鼻咽癌免疫选避有关,值得进一步研究.

Detection of peripheral blood HLA-DR-CD33+CD14-CD11b+ MDSC in patients with nasopharyngeal carcinoma

Objective To explore the distribution proportion of peripheral blood HLA-DR-CD33+ CD14-CD11b+ myeloidderived suppressor cells (MDSC) in the patients with nasopharyngeal carcinoma(NPC) to provide a beneficial guidance for NPC immunotherapy.Methods Forty-five NPC patients and 20 healthy volunteers as the healthy control group were selected.The peripheral blood sample was collected before the first time chemotherapy and on 7 d after the second cycle induction chemotherapy,meanwhile the peripheral blood sample was collected in the healthy control group;then HLA-DR-CD33+ CD14-CD11b+ MDSC were detected by the flow cytometry.Results The peripheral blood MDSC ratio of NPC patients was significantly higher than that of healthy control group(P＜0.05).With the NPC stage Ⅱ,Ⅲ and Ⅳ increase,the peripheral blood MDSC ratio was gradually increased (P＜0.05).The MDSC ratio of NPC patients with three different pathological types of keratin type squamous carcinoma,differentiated and undifferentiated type non-keratin type squamous carcinoma was significant higher than that of healthy control group(P＜0.05).The peripheral blood MDSC ration also had statistical difference among the NPC patients with 3 kinds of different pathological type(P＜0.05).The peripheral blood MDSC ratio in NPC patients with different stages was significantly decreased after 2-cycle induction chemotherapy (P ＜ 0.05),but its ratio level was still higher than that of the healthy control group.Conclusion The peripheral blood HLA-DR-CD33+ CD14-CD11b+ MDSC ratio in the NPC patients is significantly increased,suggesting that which may be related with NPC immune escape and is worth further study.