| 利用基因芯片筛选大肠癌中细胞增殖相关基因 |
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| 引用本文： | 刘志荣,李美宁,王惠珍,张悦红,牛勃,程牛亮.利用基因芯片筛选大肠癌中细胞增殖相关基因[J].山西医科大学学报,2008,39(7):585-587. |
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| 作者姓名： | 刘志荣 李美宁 王惠珍 张悦红 牛勃 程牛亮 |
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| 作者单位： | 山西医科大学生物化学与分子生物学教研室,太原,030001 |
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| 摘 要： | 目的 用基因芯片技术筛选大肠癌中细胞增殖相关基因. 方法 利用HG-U133 Plus2.0基因芯片.对大肠癌组织和正常组织基因表达谱进行检测,并利用生物信息学方法对检测结果进行分析. 结果 ①癌组织与正常组织比较,表达差异2倍以上基因共有1 200个,其中表达上调的474个,表达下调的726个.②在1 200个基因中,共有127个基因与细胞的增殖功能相关,其中表达上调的66个,表达下调的61个. 结论 127个细胞增殖相关基因可为大肠癌的基因治疗提供靶点.
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| 关 键 词： | 基因芯片 大肠肿瘤 细胞增殖相关基因 利用 基因芯片 筛选 大肠癌 细胞增殖 相关基因 gene chips colorectal cancer genes 靶点 基因治疗 功能相关 细胞的增殖 表达下调 表达上调 表达差异 比较 分析 结果 生物信息学方法 |
Screening the cell-proliferation-associated genes expressed in colorectal cancer by gene chips |
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| LIU Zhi-rong,LI Mei-ning,WANG Hui-zhen,ZHANG Yue-hong,NIU Bo,CHENG Niu-liang.Screening the cell-proliferation-associated genes expressed in colorectal cancer by gene chips[J].Journal of Shanxi Medical University,2008,39(7):585-587. |
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| Authors: | LIU Zhi-rong LI Mei-ning WANG Hui-zhen ZHANG Yue-hong NIU Bo CHENG Niu-liang |
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| Institution: | Dept of Biochemistry and Molecular Biology;Shanxi Medical University;Taiyuan 030001;China; |
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| Abstract: | Objective To screen the cell-proliferation-associated genes expressed in colorectal cancer by gene chips.Methods The HG-U133 Plus2.0 gene chips were used to analyze gene expression profiles of colorectal adenocarcinoma and normal tissues.The results were analyzed by bioinformatic techniques.Results Compared with normal tissues,a total of 1 200 genes with more than 2-fold differential expression were detected in adenocarcinoma tissues,of which,474 and 726 genes were up-regulated and down-regulated,respective... |
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| Keywords: | gene chips colorectal neoplasms cell-proliferation-associated genes |
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