宁肠汤治疗肠易激综合征的实验研究

目的:观察宁肠汤对束缚应激致泄大鼠小肠运动功能及血浆胃动素(MOT)、P物质(SP)、血管活性肠肽(VIP)的影响,并探讨其作用机理.方法:将SD雄性大鼠50只随机分为正常组,模型组,宁肠汤高、低剂量组,补脾益肠丸组,采用williams改良方法建立束缚应激致大鼠泄泻模型,观察宁肠汤对束缚应激所致大鼠排便的影响,对大鼠小肠运动功能的影响;并以放射免疫法检测各组大鼠血浆MOT、SP、VIP的变化.结果:模型组大鼠小肠墨汁推进率较正常组明显增高(P＜0.01),而各治疗组较模型组明显下降(P＜0.05);模型组大鼠血浆MOT、VIP水平较正常组明显升高(P＜0.01),SP水平较正常组明显降低(P＜0.01);而宁肠汤高、低剂量组和补脾益肠丸组血浆MOT、VIP水平较模型组明显降低(P＜0.05,P＜0.01),SP水平较模型组明显升高(P＜0.05,P＜0.01).结论:宁肠汤能抑制束缚应激大鼠亢进的小肠蠕动,对束缚应激致泄泻大鼠胃肠激素有调节作用,其中能升高血浆SP,降低血浆MOT、VIP水平.

Experimental Research on Treating Irritable Bowel Syndrome with Bowel-stabilizing Decoction

Objectives:To Study the effect of the bowel stabilizing decoction(BSD) on the function of small intestinal movement,changes of motilin(MOT),substance(SP),vasoactive intestinal peptide(VIP)in the plasma of restraint stressed diarrhea rats,and explore the mechanisms of it.Methods:In study,diarrhea rats induced by restraint stress were treated with BSD,spleen strengthening and bowel benefiting pill(SSBBP) taken as treatment control group.The influences of BSD on bowel movements and moving function of the small intestine in restraint stress induced diarrhea rats were investigated and the changes of the plasma MOT,SP,VIP in these rats were examined with radio immunological assay.Results:Hyperfunction of the small intestinal movement was found in restraint stress rat group,the liquid ink pushing rate being faster than intact rats,while that in all the treated rats were similar to the intact rats.The level of plasma MOT,VIP in restraint stress rats was greatly higher than that of intact rats and the level of SP was greatly lower than that of intact rats,while the levels of plasma MOT,VIP in three treatment groups were lower than the restraint stress rat control group and the level of SP was higher than that of intact rats. Conclusions:BSD is able to inhibit the hyperactivated peristalsis of the small intestine in rats induced by restraint stress and regulate the gastrointestinal hormones in rats whth diarrea caused by restraint stress,of which it increased plasma SP,decreased plasma MOT and VIP levels.