uPA/uPAR在骨关节中的表达及与软骨细胞凋亡的关系

目的探讨uPA/uPAR在骨关节炎软骨中的表达及与软骨细胞凋亡的关系。方法成年新西兰大白兔24只,根据文献采用石膏固定方法制作骨关节炎动物模型,用免疫组化、原位末端标记技术(TUNEL)等方法检测uPA/uPAR在兔膝关节软骨中的表达和软骨细胞凋亡情况。结果在软骨中uPA/uPAR的表达实验组阳性率高于对照组(P<0.05);实验组软骨细胞凋亡程度与uPA/uPAR的表达均呈正相关(P<0.05)。结论uPA/uPAR与骨关节炎发生发展有密切关系。

The research of expression and significance of uPA/uPAR in synovium of osteoarthritic animal model

Objective] To investigate the expression and significance of uPA/uPAR in articular chondrocytes of osteoarthritic animal model. Methods] The left knees of 24 rabbits were immobilized in full extension using plaster cast. Immunohistochemistry and TUNEL assay was used to detect the expression of uPA/uPAR in articular chondrocytes and the apoptosis of the articular chondrocytes of animal model. Results] The positive expression rat of uPA/uPAR in osteoarthritic articular chondrocytes were higher than that in normal(P <0.05). There were positive correlation between degrees of the expression of uPA/uPAR and chondrocytes apoptosis in osteoarthritic articular cartilage (P <0.05). Conclusion] There was a consanguineous correlation between degrees of the expression of uPA/uPAR and chondrocytes apoptosis in osteoarthritic articular cartilage. It demonstrated that the abnormal expression of uPA/uPAR closely related to the biological behaveior of OA such as occurrence, progress, etc.