酒精处理大鼠血压、体重及主要脏器重量系数的测定

目的 提供酒精处理后大鼠血压、体重、主要脏器重量及其重量系数的数据.方法 雄性SPF级SD大鼠分为空白组、酒精低剂量组、酒精中剂量组、酒精高剂量组.酒精低剂量组给予52％酒精0.25 mL/（100 gbw·d）灌胃；酒精中剂量组给予52％酒精0.5 mL/（100g bw·d）灌胃；酒精高剂量组给予52％酒精1.0 mL/（100 gbw·d）灌胃.在灌胃4、8、12周后分别观察大鼠的血压、体重、主要脏器重量及重量系数变化情况.结果 SD大鼠在本实验酒精剂量和方式的干预下,灌胃4周后、8周后、12周后血压值均未见明显改变.灌胃4周后,酒精高剂量组大鼠的体重、心脏重量、肝脏重量、肾脏重量与空白组比较差异有显著性（P＜0.05）；酒精中剂量组大鼠的心脏重量系数与空白组比较差异有显著性（P＜0.05）.在灌胃12周后,酒精各剂量组大鼠的体重、肝脏重量系数与空白组比较差异均有显著性（P＜0.05）；酒精高剂量组大鼠的肾脏重量与空白组比较差异有显著性（P＜0.05）.结论 初步建立酒精处理大鼠一定时间后大鼠血压、体重及主要脏器重量及其重量系数的常规生理学数据,为相关的实验研究提供基础资料.

Influences of alcohol on the blood pressure and weight and coefficient of the main organs of SD rats

Objective To observe parameters blood pressure, weight, organic weight and coefficient of the main organs on SD rats by alcohol interference. Methods Healthy Sprague-Dawley male rats were randomly divided into the blank group, the low dose alcohol group, the middle dose alcohol group and the high dose alcohol group. The low dose alcohol group, the middle dose alcohol group and the high dose alcohol group were respectively administered with 0.25 mL/ （100g bw·d） , 0.5 mL/（ 100 g bw·d） , 1.0 mL/（ 100 g bw·d） 52% alcohol through tube for 4, 8 and 12 consecutive weeks. During which time we recorded changes in blood pressure, weight, organic weight and coefficient of the main organs. Results Analysis d variance revealed no significant differences on blood pressure after 4 weeks, 8 weeks and 12 weeks of the SD rat induced by three doses of alcohol. After 4 weeks, there were significant difference in weight, heart weight, liver weight and kidney weight between the high dose alcohol group and the blank group （ P 〈 0. 05 ） ; There were difference in coefficient of heart weight between the middle dose alcohol group and the blank group （ P 〈 0.05 ）. After 12 weeks, there were difference in weight and coefficient of liver weight between the alcohol group and the blank group （ P 〈O. 05 ） ; There were difference in kidney weight between the high dose alcohol group and the blank group （ P 〈 O. 05 ）. Conclusions Biological parameters of SD rats with alcohol interference and normal SD rats are established which provide basic data for further study.