培养法和PCR法用于实验大、小鼠细菌检测的比较分析

目的 比较培养法和PCR法对实验大、小鼠的细菌检测效果。方法 分别采用传统细菌分离培养结合生化鉴定方法和PCR方法, 对78只SPF级大鼠和422只SPF级小鼠进行检测, 对两种方法的检测结果进行比较分析。结果 78只SPF级大鼠均未检测出阳性。422只SPF级小鼠, 培养法检测阳性率7.11%(30/422), 其中金黄色葡萄球菌10只, 绿脓杆菌22只, 肺炎克雷伯杆菌2只。PCR法检测阳性率7.58%(32/422), 其中金黄色葡萄球菌10只, 绿脓杆菌25只, 肺炎克雷伯杆菌2只。结论 PCR法的敏感性高于培养法, 操作简便、快捷, 适用于实验动物细菌的快速诊断和大规模的质量筛查。

Comparison of culture and PCR assay for detection of bacteria in laboratory rats and mice

Objective To compare the efficiency of bacteria culture and PCR assays for detection of Staphylococcus aureus (S. aureus), Pseudomonas aeruginosa (P. aeruginosa) and Klebsiella pneumoniae (K. pneumoniae) in laboratory rats and mice. Methods Bacteria culture combined with biochemical identification and PCR assay were used to detect 78 SPF rats and 422 SPF mice and the results of the two methods were compared. Results All the 78 rats were negative. Of the 422 mice, the positive rate by culture was 7.11% (30/422), of which, 10 were S. aureus, 22 were P. aeruginosa, and 2 were K. pneumoniae. The positive rate by PCR was 7.58% (32/422), of which, 10 were S. aureus, 25 were P. aeruginosa, and 2 were K. pneumoniae. Conclusions The high sensitivity, rapid procedure and easy to operate of PCR assay makes it valuable for rapid bacteria diagnosis and large-scale screening in laboratory animals.