不同周龄BWEL-SPF种鸡生理生化指标的测定

目的测定不同周龄的BWEL-SPF种鸡生理和生化指标,并对测定值进行t-检验。方法分别采用计数板法、血涂片法、血液全自动分析仪测定法、双缩尿法、溴甲酚绿法、酶比色法和硫氰酸汞比色法等测定了10、40和60周龄的雌、雄BWEL-SPF种鸡的生理生化指标,并对雌、雄差异进行了统计学分析。结果在所测定的生理生化指标中,不同周龄雌雄之间差异显著性表现项目不同,其中在10周龄时,只有总胆固醇(CHO)在雌、雄之间差异极显著(P<0.01);40周龄时,甘油三酯(TG)、在雌、雄之间差异极显著(P<0.01);总胆固醇(CHO)为雌、雄之间差异显著(P<0.05);60周龄时,出血时间(BT)、嗜碱性粒细胞(B)、单核细胞(M)、谷丙转氨酶(ALT)、葡萄糖(Glu)在雌、雄之间差异极显著(P<0.01),而凝血时间(CT)和甘油三酯(TG)在雌、雄之间差异显著(P<0.05),除此以外,其它生理生化指标在雌雄之间差异不显著。结论BWEL-SPF种鸡的生理和生化指标随周龄不同,在雌、雄之间的差异随之变化。

Determination of Values of Physiological and Biochemical Parameters in BWEL-SPF Chickens at Age of 10,40 and 60 Weeks

Objective To determine the values of physiological and biochemical parameters in BWEL-SPF chickens at age of 10,40 and 60 weeks.Methods The values of T,P,BT,CT,ESR,systolic pressure,diastolic pressure,RBC,HCT,MCV,RDW,HGB,MCH,MCHC,PLT,MPV,WBC,N,E,B,L,M,TP,Alb,AST,ALT,Glu and TG and CHO in BWEL-SPF chickens at age of 10,40 and 60 weeks,both male and female,were determined by corresponding measurement and t-test was made to analyze the results.Results There was a significant difference between CHO values in male and female chickens at age of 10 weeks(P<0.01),a very significant difference between TG,Ca,Na and Mg values in male and female chickens at age of 40 weeks(P<0.01),a significant difference between CHO values in male and female chickens at age of 40 weeks(P<0.05),a very significant difference between BT,B,M,ALT and Glu values in male and female chickens at age of 60 weeks(P<0.01),and a significant difference between CT and TG values in male and female chickens at age of 60 weeks(P<0.05).Conclusion The values of the physiological and biochemical parameters were different between male and female BWEL-SPF chickens at age of 10,40 and 60 weeks.