四物汤改善多囊卵巢综合征大鼠卵巢功能实验研究

目的观察四物汤(Siwu tang,SWT)对多囊卵巢综合征(polycystic ovarian syndrome,PCOS)大鼠动情周期、卵巢形态学及血清激素水平的影响。方法选取SPF级雌性Wistar大鼠25只,随机分为对照组、模型组、SWT低剂量组、SWT高剂量组和二甲双胍组,每组5只。除对照组外,各实验组大鼠均采用来曲唑1 mg/(kg·d)灌胃诱导建立PCOS大鼠模型。造模成功后,SWT低剂量组给予SWT 8.64 g/(kg·d)灌胃,SWT高剂量组给予SWT 25.92 g/(kg·d)灌胃,二甲双胍组给予二甲双胍200 mg/(kg·d)灌胃,对照组与模型组灌胃等量0.9%氯化钠注射液。各组均连续干预4周后,记录各组大鼠的动情周期,观察卵巢形态,检测血清激素水平。结果与对照组相比,模型组大鼠动情期显著缩短(P<0.05);黄体数量显著减少,囊肿数量显著增多(P<0.05);血清睾酮(testosterone,T)、黄体生成素(luteinizing hormone,LH)水平显著升高,雌二醇(estradiol,E2)、卵泡刺激素(follicle-stimulating hormone,FSH)水平显著降低,LH/FSH比值明显升高(P<0.05)。与模型组大鼠相比,SWT低剂量组、SWT高剂量组和二甲双胍组大鼠动情期时间显著增加(P<0.05);黄体数量显著增加,囊肿数量显著减少(P<0.05);血清T水平及LH/FSH比值显著降低(P<0.05);SWT低剂量组、SWT高剂量组大鼠血清E2水平显著升高(P<0.05);SWT高剂量组大鼠血清FSH水平显著升高、LH水平显著降低(P<0.05)。结论SWT可增加PCOS大鼠动情时间,减少卵巢囊肿,增加黄体数量,还可降低PCOS大鼠血清T、LH水平及LH/FSH比值,升高E2、FSH水平;其治疗机制可能与通过调节血清激素水平改善PCOS大鼠卵巢功能有关。

Experimental Study of Siwu Tang on the Improvement of Ovarian Function in Rats with Polycystic Ovary Syndrome

Objective To observe the effect of Siwu Tang(SWT)on the estrous cycle,ovarian morphology and serum hormone levels in polycystic ovary syndrome(PCOS)rats.Methods Twenty-five SPF female Wistar rats were randomly divided into control group,model group,SWT low-dose group,SWT high-dose group and metformin group,with 5 rats in each group.Except for the control group,rats in each experimental group were gavage with 1 mg/(kg·d)letrozole to establish PCOS rat models.After successful modeling,the rats in SWT low-dose group were gavage with 8.64 g/(kg·d)SWT,SWT high-dose group were gavage with 25.92 g/(kg·d)SWT,metformin group were gavage with 200 mg/(kg·d)metformin,while the control and model group were gavage with the same dose of 0.9%NaCl injection.After 4 weeks of continuous intervention in each group,the estrus cycle of each group were recorded,the ovarian morphology were observed,and the serum hormone level were detected.Results Compared with control group,the time of estrus in the model group were significantly shortened(P<0.05),the number of corpus luteum were significantly decreased,the number of cysts were significantly increased(P<0.05),the serum levels of testosterone(T),luteinizing hormone(LH)and the LH/FSH ratio were significantly increased,the levels of estradiol(E2)and follicle-stimulating hormone(FSH)were significantly reduced.Compared with the rats in the model group,the time of estrous in the SWT low-dose group,SWT high-dose group and metformin group were significantly increased(P<0.05),the number of corpus luteum were significantly increased,and the number of cysts were significantly reduced(P<0.05),the serum levels of T and the LH/FSH ratio were significantly decreased(P<0.05).The serum levels of E2 rats in the SWT low-dose group,SWT high-dose group were significantly increased(P<0.05).The serum levels of FSH in the SWT high-dose group were significantly increased,and levels of LH were significantly decreased(P<0.05).Conclusion SWT could increase the time of estrus,reduce ovarian cysts,increase the number of corpus luteum,reduce the serum levels T,LH and LH/FSH ratios,and increase E2 and FSH levels in PCOS rats.The therapeutic mechanism might be related to improvement of ovarian function in PCOS rats by regulating serum hormone levels.