新藤黄酸对人乳腺癌耐药细胞体外作用的研究

目的 观察新藤黄酸（gambogenic acid,GNA）对人乳腺癌耐药细胞MCF-7/ADR的作用.方法 采用MTT法观察GNA、阿霉素（adriamycin,ADR）及两者联用对MCF-7/ADR细胞增殖的抑制作用,采用流式细胞仪观察MCF-7/ADR细胞内ADR浓度.结果 GNA能剂量依赖地抑制MCF-7/ADR细胞的生长,作用48 h时,GNA对MCF-7/ADR细胞的IC50为12.88 μmol/L;4 μmol/L GNA可增加MCF-7/ADR细胞对ADR的敏感性,使ADR对MCF-7/ADR细胞的IC50由80.22 μmol/L降至12.54 μmol/L;GNA显著增强MCF-7/ADR细胞内ADR的积累,呈剂量依赖关系.结论 低剂量GNA能显著增加MCF-7/ADR细胞对ADR的敏感性.

In Vitro Effect of Gambogenic Acid on Multidrug-resistant Human Breast Cancer Cells

Objective To observe the effect of gambogenic acid （GNA） on multidrug resistant human breast cancer cells （MCF-7/ADR）.Methods MTT assay was used to assess the inhibitory effect of GNA,adriamycin （ADR）,or a combination of GNA and ADR on the proliferation of MCF-7/ADR cells.Flow cytometry was used to determine the concentration of ADR in MC-F 7/ADR cells.Results GNA suppressed the proliferation of MCF-7/ADR cells in a dose dependent manner,and the half maximal inhibitory concentration （IC50） for MCF-7/ADR cells was 12.88 μmol/L at 48 h; GNA increased the sensitivity of MCF-7/ADR cells to ADR at a dose of 4μmol/L,so that the IC50 of ADR for MCF-7/ADR cells decreased from 80.22 μmol/L to 12.54 μmol/L; GNA also significantly increased the intracellular accumulation of ADR in MCF 7/ADR cells in a dose dependent manner.Conclusion GNA can significantly increase the sensitivity of MCF-7/ADR cells to ADR at a low dose.