| 醛固酮对大鼠肾小球系膜细胞表达结缔组织生长因子的影响 |
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| 引用本文： | 白寿军,张永,张建鄂,李彩霞,徐钢,刘晓城.醛固酮对大鼠肾小球系膜细胞表达结缔组织生长因子的影响[J].郧阳医学院学报,2009,28(2):131-133. |
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| 作者姓名： | 白寿军 张永 张建鄂 李彩霞 徐钢 刘晓城 |
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| 作者单位： | 白寿军,BAI Shou-jun(华中科技大学附属同济医院肾内科,湖北,武汉,430030;郧阳医学院附属太和医院肾内科,湖北,十堰,442000);张永,张建鄂,ZHANG Yong,ZHANG Jia-ne(郧阳医学院附属太和医院肾内科,湖北,十堰,442000);李彩霞,徐钢,刘晓城,LI Cai-xia,XU Gang,LIU Xiao-cheng(华中科技大学附属同济医院肾内科,湖北,武汉,430030)
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| 摘 要： | 目的:探讨醛固酮(ALD)对大鼠肾小球系膜细胞表达结缔组织生长因子(CTGF)的影响,及转化生长因子β1(TGF-β1)是否参与其信号通路。方法:大鼠肾小球系膜细胞加入不同浓度的ALD共同培养0、12、24、36、48h,RT-PCR方法评价CTGF mRNA的表达;用ELISA方法检测TGF-β1蛋白的表达;并用TGF-β1/Smads信号通路的特异性丝、苏氨酸激酶抑制剂Staurosporine(STA)加以对比。结果:ALD刺激后肾小球系膜细胞CTGF mRNA表达水平增加,且呈剂量依赖性,TGF-β1蛋白表达也明显增加;用STA预处理1h后再用ALD刺激24h,则CTGFmR-NA的表达无明显变化。结论:ALD通过TGF-β1/Smads途径上调肾小球系膜细胞表达CTGF。
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| 关 键 词： | 醛固酮 结缔组织生长因子 转化生长因子β1 肾小球系膜细胞 信号途径 |
Effect of Aldosterone on the Expression of Connective Tissue Growth Factor in Cultured Rat Mesangial Cells |
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| BAI Shou-jun,ZHANG Yong,ZHANG Jia-ne,LI Cai-xia,XU Gang,LIU Xiao-cheng.Effect of Aldosterone on the Expression of Connective Tissue Growth Factor in Cultured Rat Mesangial Cells[J].Journal of Yunyang Medical College,2009,28(2):131-133. |
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| Authors: | BAI Shou-jun ZHANG Yong ZHANG Jia-ne LI Cai-xia XU Gang LIU Xiao-cheng |
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| Institution: | BAI Shou-jun,ZHANG Yong, ZHANG Jian-e, LI Cai-xia, XU Gang, LIU Xiao-cheng (Department of Nephrology, 1Tongfi Hospital ,Huazhong University of Science and Technology, Wuhan , Hubei 430030 ;2 Taihe Hospital, Yunyang Medical College ,Shiyan ,Hubei 44200, China) |
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| Abstract: | Objective To explore the effect of aldosterone on the expression of CTGF in cultured rat mesangial ceils, and whether TGF-β1 was involved in the signaling pathway. Methods Rat glomerular mesangial cells were incubated with aldosterone at different concentrations for 0,12,24,36,48 h,respectively. The expression of CTGF mRNA was determined with RT-PCR, and the expression of TGF-β1 protein was assessed with ELISA. Staurosporine, a Serine/Threonine kinase inhibitor of TGF-β1/Smads signaling pathway,was used to inhibit CTGF expression. Results The level of CTGF mRNA was significantly increased after exposure to aldosterone, and it was dose-dependent, the expression of TGF-β1 protein was also signifi- cantly increased. However, aldosterone could not stimulate the expression of CTGF mRNA with staurosporine pre-treament for 1 h. Conclusion Aldosterone could upregulate the expression of CTGF in cultured rat mesangial cells through TGF-β1/ Smads signaling pahtway. |
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| Keywords: | Aldosterone Connective tissue growth factor Transforming growth factor-β1 Glomerular mesangial cell Signa- ling pathway |
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