主动脉缩窄术模型小鼠成模早期评价技术研究

目的 主动脉缩窄小鼠是常用的心肌病模型，但是很多研究者在使用该模型时会遇到判定模型成功与否的问题。本文结合国内外该模型的建立与评价技术，利用显微手术建立主动脉缩窄小鼠模型，并利用超声多普勒技术分析建立该模型早期评价的方法。方法 C57BL/6J小鼠经开胸手术结扎主动脉弓，获得理论直径为0.4mm的无名动脉远端主动脉弓重建血管；超声多普勒分析重建小鼠主动脉弓及其分支内血流流速，分析小鼠主动脉重建情况；记录小鼠生存情况，Spss16.0软件Kaplan-Meier curves分析小鼠存活率；将术后1周多普勒血流分析数据与术后4周左室壁厚度做相关性分析；生物信息学总结分析国内外立该模型的技术与评价，综合分析建立主动脉缩窄小鼠模型的超声多普勒早期评价方法，为相关研究者提供参考。 结果 术后8周时重建组（TAC）小鼠存活率为85.0%（n=40），而假手术组（Sham）小鼠存活率为96.4%（n=28）；小鼠术后1周超声多普勒检测无名动脉血流/左颈总动脉血流比值（IA/LCCA）大于5.9时，术后4周舒张期左室后壁厚度（LVPWD）显著增厚占比达100%；IA/LCCA于5.9~10.7之间时与LVPWD成线性相关，当IA/LCCA大于10.7时线性关系下降，LVPWD增大趋势变缓。结论 TAC小鼠术后1周时使用多普勒血流分析，IA/LCCA比值大于5.9时，术后4周小鼠LVPWD即发生显著增厚，超声多普勒血流分析可以作为模型是否成功的早期评价方法。

The study of evaluation technology at early stage on transverse aortic constriction mice model

Objective Mice model by transverse aortic constriction (TAC) was commonly used in cardiomyopathy studies. But the evaluation of successful ligation is a problem. In this paper, we established the TAC mice model consulting references about this technique at home and abroad, and use Doppler as a early stage evaluation technology. Methods The 0.4 mm reconstructed aortic was established theoretically, and the flow of aorta and branches were analysed bydoppler analysis. The mortality of TAC mice was calculated and analyzed by the Spss 16.0 software. The dependablity between doppler analysis on flow 1 week after reconstruction and echocardiography analysis on left ventricle (LV) wall thickness 4 weeks after reconstruction was essaied. This technology was evaluated on references at home and abroad. Reasults The survival rate was 85% (n=40) 8 weeks after reconstriction, while it was 96.4% in the sham group (n=28). The LV posterior wall at end-diastole (LVPWD) will give significance when the IA/LCCA flow ratio>5.9. The IA/LCCA flow ratio will give liner correlation to LVPWD when it is between 5.9 and 10.7, and the liner correlation will decreased when the ratio>10.7. Conclusions The LVPWD will give significant thickness 4 weeks after reconstruction when the IA/LCCA flow ratio>5.9 1 week after reconstruction, and the Doppler analysis can be used as early stage evaluation technology on transverse aortic constriction mice model.