| 黄连素降糖调脂机制的研究 |
| |
| 引用本文： | 张茜,肖新华,王彤,朱席琳,李文慧,刘英,袁涛,孙晓方,孙琦,向红丁,王姮.黄连素降糖调脂机制的研究[J].实验动物与比较医学,2011,19(1). |
| |
| 作者姓名： | 张茜 肖新华 王彤 朱席琳 李文慧 刘英 袁涛 孙晓方 孙琦 向红丁 王姮 |
| |
| 作者单位： | 1. 中国医学科学院,北京协和医学院,北京协和医院内分泌科,北京,1007302. 中国医学科学院基础医学研究所,北京协和医学院基础学院医学分子生物学国家重点实验室,北京,100730 |
| |
| 摘 要： | 目的 旨在研究黄连素对KKAy小鼠体重、血糖、血脂、胰岛素等相关代谢指标的影响,且应用基因芯片探讨黄连素降血糖、调血脂的机制.方法 选用KKAy小鼠16只,随机分为黄连素组(给予每日250 mg/kg的黄连素粉末悬浊液)和对照组(给予等体积生理盐水),均连续灌胃4周.每周末测定小鼠空腹血糖(FBG)和体重.3周末进行口服糖耐量(OGTT)实验.4周末测定小鼠FBG、血脂和空腹胰岛素(FINS)水平.取小鼠骨骼肌组织进行RT-PCR基因芯片实验.结果黄连素组小鼠FBG、OGTT曲线下面积(AUC)、TC、TG、FINS和HOMA-IR值均比对照组显著降低(P<0.05或P<0.01).基因芯片显示黄连素组GLUT4、MAPK8、MAPK14、PPARα、UCP2上调,PPARγ、PGC、CEBP、resistin、HNF4α下调.结论 黄连素不仅能有效降低KKAy小鼠FBG,改善胰岛素敏感性,还能调节脂代谢.黄连素可能是通过AMPK-p38 MAPK-GLUT4通路调节KKAy小鼠血糖.PPARα上调参与黄连素对KKAy小鼠血脂的调节.
|
| 关 键 词： | 黄连素 KKAy小鼠 基因芯片 糖尿病 脂代谢紊乱 糖代谢 |
Mechanism of berberine regulating glucose and lipid metabolism studied with RT PCR array |
| |
| ZHANG Qian,XIAO Xin-hu,WANG Tong,ZHU Xi-lin,LI Wen-hui,LIU Ying,YUAN Tao,SUN Xiao-fang,SUN Qi,XIANG Hong-ding,WANG Heng.Mechanism of berberine regulating glucose and lipid metabolism studied with RT PCR array[J].Laboratory Animal and Comparative Medicine,2011,19(1). |
| |
| Authors: | ZHANG Qian XIAO Xin-hu WANG Tong ZHU Xi-lin LI Wen-hui LIU Ying YUAN Tao SUN Xiao-fang SUN Qi XIANG Hong-ding WANG Heng |
| |
| Institution: | ZHANG Qian1,XIAO Xin-hua1,WANG Tong1,ZHU Xi-lin2,LI Wen-hui1,LIU Ying2,YUAN Tao1,SUN Xiao-fang1,SUN Qi1,XIANG Hong-ding1,WANG Heng1(1.Department of Endocrinology,Peking Union Medical College Hospital,Peking Union Medical College,Chinese Academy of Medical Sciences,Beijing 100730,China,2.National Laboratory of Medical Molecular Biology,Institute of Basic Medical Sciences,School of Basic Medicine,Beijing 100730) |
| |
| Abstract: | Objective To explore the effects of berberine on the weight,blood glucose,lipid metabolism and ser-um insulin in KKAy mice and to investigate its possible glucose-lowing and lipid-regulating mechanism.Methods KKAy mice were randomly divided into two groups: berberine group(treated with 250 mg /kg /d berberine,n = 8) and control group(n = 8).The fasting blood glucose,weight,TC,TG,HDL-c,LDL-c and fasting serum insulin were tested.The OGTT test was done.RT PCR array experiment was done using skeletal muscle of... |
| |
| Keywords: | Berberine KKAy mice RT PCR array Diabetes mellitus Lipid metabolic disorders Glucose me-tabolism |
|
| 点击此处可从《实验动物与比较医学》浏览原始摘要信息 |
| 点击此处可从《实验动物与比较医学》下载免费的PDF全文 |
|
