护骨素基因启动子区T950C多态性与2型糖尿病合并骨质疏松症的关系

  目的  探讨护骨素（OPG）基因启动子区T950C的多态性与2型糖尿病（T2DM）合并骨质疏松症的关系，了解T2DM合并骨质疏松症与遗传相关的易感基因。  方法  （1）使用聚合酶链反应技术，检测T2DM不合并骨质疏松组（A组），T2DM合并骨量减少组（B组），T2DM合并骨质疏松组（C组），OPG基因T950C的基因型，分别分析3组的OPG基因T950C多态性；（2）比较3组人群性别、体重指数、年龄、血压、体重、糖化血红蛋白、身高、血脂、空腹血糖、睾酮、雌激素、维生素D等临床指标；（3）比较OPG基因T950C的基因型间不同部位的骨密度差异；（4）通过Logistic回归分析了解T2DM合并骨质疏松患者的独立危险因素。  结果  （1）TC型是T2DM和T2DM合并骨质疏松症人群OPG基因T950C的主要基因型；（2）3组OPG基因T950C基因型的频率及等位基因频率的差异均无统计学意义（P > 0.05）；（3）3组患者体重、年龄、身高、BMI、比较，差异有统计学意义（P < 0.05），且各组间相互比较，差异有统计学意义（P < 0.05）；（4）OPG基因T950C的3种基因型比较，各部位的骨密度均无差异（P > 0.05）；（5）回归分析显示:年龄、吸烟可进入回归方程，而OPGOPG基因T950C的基因型未进入回归方程。  结论  （1）TC型是T2DM和T2DM合并骨质疏松症患者OPG基因T950C的主要基因型；（2）OPG基因T950C的基因型可能不是T2DM及T2DM合并骨质疏松症的易感基因；（3）吸烟和年龄是T2DM合并骨质疏松患者的独立危险因素。

The Relationship Between T950C Polymorphism in the Promoter Region of Osteoprotegerin Gene and Type 2 Diabetes with Osteoporosis

  Objective  To investigate the relationship between T950C polymorphism in the promoter region of osteoprotegerin （OPG） gene and type 2 diabetes mellitus （T2DM） complicated with osteoporosis, and to understand the genetically related susceptibility genes of T2DM complicated with osteoporosis.   Methods   （1） Using polymerase chain reaction technology, the T2DM without osteoporosis group （group A）, the T2DM with osteopenia group （B group）, the T2DM with osteoporosis group （C group）, the OPG gene T950C group were detected. Genotype, the OPG gene T950C polymorphism in the three groups was analyzed respectively; （2） Gender, body mass index, age, blood pressure, weight, glycated hemoglobin, height, blood lipids, fasting blood glucose, testosterone, estrogen, vitamin D were compared among the three groups. （3） The differences in bone mineral density was compared between different parts of the OPG gene T950C genotype; （4） The independent risk factors of T2DM patients with osteoporosis was learned through logistic regression analysis.   Results   （1） TC type was the main genotype of OPG gene T950C in T2DM and T2DM patients with osteoporosis; （2） There was no significant difference in the genotype frequency and allele frequency of OPG gene T950C among the three groups （P > 0.05）; （3） The weight, age, height, BMI, and BMI of the three groups of patients were compared, the difference was statistically significant （P < 0.05）, and the differences among the groups were statistically significant （P < 0.05）; （ 4） Comparison of the three genotypes of the OPG gene T950C, there was no difference in the bone mineral density of each part （P > 0.05）; （5） Regression analysis showed that age and smoking entered the regression equation, but the genotype of the OPGOPG gene T950C did not enter regression equation.   Conclusions   （1） TC type is the main genotype of OPG gene T950C in T2DM and T2DM patients with osteoporosis; （2） The genotype of OPG gene T950C may not be a susceptibility gene for T2DM and T2DM combined with osteoporosis; （3） Smoking and age are independent risk factors of T2DM combined with osteoporosis.