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| 异鼠李素对HeLa细胞端粒酶活性的抑制作用 | |
| 引用本文： | 杨春蕾,屈艺,王正荣,陶大昌.异鼠李素对HeLa细胞端粒酶活性的抑制作用[J].四川大学学报(医学版),2004,35(2):198-200. |
| 作者姓名： | 杨春蕾 屈艺 王正荣 陶大昌 |
| 作者单位： | 1. 四川大学生命科学院,医学细胞生物学教研室,成都,610041 2. 四川大学基础医学与法医学院分子生物学与生物化学教研室 3. 四川大学基础医学与法医学院,生物医学工程研究室 |
| 基金项目： | 国家自然科学基金 (批准号 3 9970 2 75 ,3 0 0 70 2 78)资助 |
| 摘 要： | 目的 观察异鼠李素对 He L a细胞生长的抑制和诱导凋亡的作用 ,并探讨端粒酶在此过程中的作用。方法 培养细胞加入异鼠李素 ,通过细胞增殖动力学、流式细胞术及 TRAP- EL IAS法检测异鼠李素对 He L a细胞凋亡及端粒酶活性的影响。结果 异鼠李素处理组细胞生长受到抑制 ,抑制作用均与浓度呈梯度关系 ,其 IC50 为(4 0± 0 .0 8) μg/ m l,端粒酶活性较对照组细胞降低 (P<0 .0 5 )。结论 异鼠李素可通过抑制 He L a细胞端粒酶活性而抑制肿瘤细胞的生长 |
| 关 键 词： | 异鼠李素 凋亡 端粒酶 端粒 永生化 |
| 修稿时间： | 2003年7月23日 |
Inhibitory Effect of Isorhmnetin on Telomerase Activity of HeLa Cells |
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| Chun-lei Yang,Yi Qu,Zheng-rong Wang,Da-chang Tao.Inhibitory Effect of Isorhmnetin on Telomerase Activity of HeLa Cells[J].Journal of West China University of Medical Sciences,2004,35(2):198-200. | |
| Authors: | Chun-lei Yang Yi Qu Zheng-rong Wang Da-chang Tao |
| Institution: | Department of Cell Biology, Life Science College, Sichun University, Chengdu 610041, China. |
| Abstract: | OBJECTIVE: To investigate the growth-inhibiting and apoptosis-inducing effects of isorhmnetin on HeLa cells and to disclose the role of telomerase activity of tumor cells. METHODS: The methods of cell culture in vitro were adopted. HeLa cells were treated with isorhmnetin in different concentrations for 2 days, and then were observed and analyzed by use of MTT, Flow-Cytometry (FCM) and TRAP-ELIAS technique for inspecting the HeLa cells' growth and telomerase activity. RESULTS: The growth of HeLa cells was inhibited evidently after treatment by isorhmnetin. The rate of apoptosis was 31.7% after the HeLa cells were treated with 20 micrograms/ml isorhmnetin. Isorhmnetin could inhibit the activity of telomerase. CONCLUSION: By inhibiting the activity of telomerase, isorhmnetin can inhibit the growth of HeLa cells. |
| Keywords: | Isorhmnetin Apoptosis Telomerase Telomere Immortality |
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