吴茱萸透皮贴剂贴敷神阙穴对营养性肥胖小鼠脂质代谢及肠道菌群的影响

目的:探讨吴茱萸透皮贴剂贴敷神阙穴对营养性肥胖鼠脂质代谢及肠道菌群的影响。方法:复制营养性肥胖模型,随机分为正常组、模型组、药贴14d组和药贴28d组,观察各组小鼠体质量、Lee’s指数、血清血脂(CHO、TG)、脂肪细胞因子(LP、ADPN)、脂肪酸合成酶(FAS)及肠道菌群的变化。结果:模型组体质量、Lee’s指数、CHO、TG、LP、ADPN及脂肪和肝脏组织中FAS含量均显著高于正常组(P<0.01),经两个药贴组治疗后上述各项指标均较模型组显著下降(P<0.01,P<0.05),且药贴28d组优于药贴14d组(P<0.05)。两个药贴组肠道蓝藻菌门和纤维杆菌门丰度显著提升(P<0.01),药贴14d组较药贴28d组变化明显(P<0.05)。结论:通过吴茱萸透皮贴剂贴敷神阙穴可改善营养性肥胖鼠的血脂异常,良性调控肥胖鼠肠道菌群的结构,达到减肥效果。

Effects on lipid metabolism and intestinal flora in high-fat diet obese mice by tetradium ruticarpum transdermal patchs on Shenque(CV 8)

Objective:To investigate the effects on the lipid metabolism and intestinal flora of nutritional obese mice of tetradium ruticarpum transdermal patchs in Shenque(CV 8).Methods:Replicated the high-fat-diet induced obesity mice,randomly selected in normal group.After 8 weeks with feeding high-fat diet,18 obese mice that the weight was greater than 20%of the normal group were randomly divided into model group,Patch 14 d group and Patch 28 d group.Then,the body weight,Lee’s index,serum lipids(CHO,TG),adipokines(LP,ADPN),and fatty acid synthase(FAS)in fat and liver tissues of experimental mice in each group were observed,as well as the changes in the intestinal flora.Results:The weight,Lee’s index,content of CHO,TG,LP,ADPN and FAS were significantly higher than those in the normal group(P<0.01).All of them were significantly decreased after tetradium ruticarpum transdermal patchs in Shenque(CV 8)(P<0.01,P<0.05),and the Patch 28 d group was significantly better than the Patch 14 d group(P<0.05).The structure and abundance in Cyanobacteria and Fibrobacteres of intestinal flora in Patch 14 d group and Patch 28 d group were significantly increased than M group(P<0.01),and the changes in the Patch 14 d group were more obvious than Patch 28 d group(P<0.05).Conclusion:Through tetradium ruticarpum transdermal patchs in Shenque(CV 8)can improve the dyslipidemia and regulate the structure and function of intestinal flora of obese mice and to achieve the goal for losing weight.