| HPLC测定左归丸中齐墩果酸与熊果酸含量 |
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| 引用本文： | 张汶婕,管大平,钱世兵.HPLC测定左归丸中齐墩果酸与熊果酸含量[J].中国实验方剂学杂志,2012,18(5):92-94. |
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| 作者姓名： | 张汶婕 管大平 钱世兵 |
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| 作者单位： | 安徽省铜陵市食品药品检验所,安徽铜陵,244000 |
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| 摘 要： | 目的:建立HPLC测定左归丸中齐墩果酸、熊果酸含量的方法.方法:采用反相高效液相色潜法,Sunfire C18柱,甲醇-水(85∶ 15)(磷酸二氢钠调pH 6.5),流速1.0 mL· min-1,检测波长215 nm,柱温25℃.结果:齐墩果酸在0.05 ～1.0 μg线性关系良好(r=0.9999),回收率97.7％( RSD 1.39％,n=6);熊果酸在0.1～2.0 μg线性关系良好(r=0.999 9),回收率98.3％( RSD 0.82,n=6).结论:方法可用于左归丸中熊果酸、齐墩果酸的含量测定.
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| 关 键 词： | 左归丸 齐墩果酸 熊果酸 高效液相色谱法 含量测定 |
| 收稿时间： | 2011/10/5 0:00:00 |
Determination of Ursolic Acid and Oleanolic Acid in Zuogui Wan by HPLC |
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| ZHANG Wen-jie,GUAN Da-ping and QIAN Shi-bing.Determination of Ursolic Acid and Oleanolic Acid in Zuogui Wan by HPLC[J].China Journal of Experimental Traditional Medical Formulae,2012,18(5):92-94. |
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| Authors: | ZHANG Wen-jie GUAN Da-ping and QIAN Shi-bing |
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| Institution: | Tongling Institute for Food and Drug Control, Tongling 244000, China;Tongling Institute for Food and Drug Control, Tongling 244000, China;Tongling Institute for Food and Drug Control, Tongling 244000, China |
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| Abstract: | Objective:To establish a HPLC method for the determination of ursolic acid and oleanolic acid in Zuogui Wan.Method: The method was applied with Sunfire C18 column by methanol-water(25∶75,adjust pH to 6.5 with sodium dihydrogen phosphate) as the mobile phase,with the flow rate 1.0 mL·min-1.The column temperature was maintained at 25 ℃ and the UV detection wavelength was set at 215 nm.Result: Oleanolic acid was linear in the range of 0.05-1.0 μg(r=0.999 9),the average recovery was 97.7%(RSD 1.39,n=6).Ursolic acid was linear in the range of 0.1-2.0 μg(r=0.999 9),the average recovery was 98.3%(RSD 0.82,n=6).Conclusion: The method can be used for determination of oleanolic acid and ursolic acid in Zuogui Wan. |
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| Keywords: | Zuogui Wan ursolic acid oleanolic acid HPLC content |
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