鸡矢藤提取物对糖尿病模型小鼠的保护作用

目的:研究鸡矢藤提取物(EPS)对链脲佐菌素(STZ)诱导的糖尿病小鼠血糖和抗氧化作用的影响。方法:小鼠尾静脉注射120 mg·kg-1STZ建立糖尿病模型。糖尿病小鼠随机分为5个组,每组10只动物,分别为:模型组,二甲双胍组,鸡矢藤低、中、高剂量组(2.5,5,10 g·kg-1),另设10只正常小鼠作为空白对照组。灌胃给药14 d,并于药前、药后7,14 d测定小鼠空腹血糖(FBG)。末次给药后取材,测定小鼠血清和肝组织中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)的活性和丙二醛(MDA)含量。结果:与空白组比较,模型组小鼠的FBG显著升高,而血清和肝组织中SOD和GSH-Px的活性显著降低,MDA含量升高。与模型组比较,鸡矢藤给药组能显著降低小鼠血糖(P0.01)。经鸡矢藤提取物治疗后,血清和肝组织中的SOD和GSH-Px活性明显升高,MDA含量有所下降(P0.05,P0.01)。结论:鸡矢藤提取物能降低糖尿病小鼠血糖,其机制可能与提高机体肝组织的抗氧化能力有关。

Protective Effect of Extracts of Paederia scandens on Streptozotocin-induced Diabetic Mice

Objective: To investigate the influence of extracts of Paederia scandens (EPS) on blood glucose and anti-oxidative effect in streptozotocin(STZ)-induced diabetic mice. Method: Diabetic mice were established by vein intravenous injection with STZ (120 mg·kg^-1). The diabetic mice were randomly divided into five groups, each group was consist of 10 mice:model group, metformin group, low-, medium-, high-dose (2.5, 5, 10 g·kg^-1) of EPS groups. Another 10 normal mice were considered as normal control group. The mice were administrated with drugs for 14 days, and the fasted blood glucose (FBG) was determined at 0^th day, 7^th day and 14^th day. After 14 days, the superoxide dismutase(SOD), malondialdehyde(MDA) and glutathione peroxidase(GSH-Px) activities in serum and liver tissue were measured. Result: Compared to normal control group, the serum and liver tissue levels of SOD and GSH-Px in model group were significantly decreased, and the FBG and MDA levels were increased. The FBG of EPS administration groups were significantly decreased compared with model group. While the serum and liver tissue levels of SOD and GSH-Px were elevated and the MDA content was decreased after EPS administration. Conclusion: The hypoglycemic mechanism of EPS on diabetic mice was likely involved in improving the anti-oxidative capability of liver tissue.